Human LAMP-1/CD107a PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC4800P
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 508921
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAMP1/CD107a
Ala28-Asn380
Accession # P11279
Ala28-Asn380
Accession # P11279
Specificity
Detects human LAMP1/CD107a in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human LAMP-1/CD107a PE-conjugated Antibody
Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry.
Enriched human NK cells either (A) treated with 1:1 ratio with K562 cell line for 5 hours or (B) untreated were stained with Mouse Anti-Human LAMP-1/CD107a PE-conjugated Monoclonal Antibody (Catalog # IC4800P) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P).
Detection of LAMP‑1/CD107a in THP‑1 Human Cell Line by Flow Cytometry.
THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP-1/CD107a PE-conjugated Monoclonal Antibody (Catalog # IC4800P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human LAMP-1/CD107a PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Enriched or expanded human NK cells cultured with 1:1 ratio of K562 cell line target cells
Sample: Enriched or expanded human NK cells cultured with 1:1 ratio of K562 cell line target cells
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: THP‑1 human acute monocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: THP‑1 human acute monocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: LAMP-1/CD107a
Lysosome-associated membrane protein-1 (LAMP1), also known as CD107a, is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP1 shares 64% aa identity with mouse LAMP1.
Long Name
Lysosome-associated Membrane Glycoprotein 1
Alternate Names
CD107a, LAMP1
Gene Symbol
LAMP1
UniProt
Additional LAMP-1/CD107a Products
Product Documents for Human LAMP-1/CD107a PE-conjugated Antibody
Product Specific Notices for Human LAMP-1/CD107a PE-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...