Human LAMP-1/CD107a APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC4800A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 508921
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAMP-1/CD107a
Ala28-Asn380
Accession # P11279
Ala28-Asn380
Accession # P11279
Specificity
Detects human LAMP-1/CD107a in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human LAMP-1/CD107a APC-conjugated Antibody
Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry.
Human NK Cells either (A) stimulated in a 1:1 ratio with K562 human chronic myelogenous leukemia cell line and 3 µM monensin for 5 hours or (B) unstimulated were stained with Mouse Anti-Human LAMP-1/CD107a APC-conjugated Monoclonal Antibody (Catalog # IC4800A) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Detection of LAMP-1/CD107a in THP‑1 Human Cell Line by Flow Cytometry.
THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP-1/CD107a APC-conjugated Monoclonal Antibody (Catalog # IC4800A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human LAMP-1/CD107a APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Enriched human NK cells incubated with K562 human chronic myelogenous leukemia cell line target cells
Sample: Enriched human NK cells incubated with K562 human chronic myelogenous leukemia cell line target cells
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: THP‑1 human acute monocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: THP‑1 human acute monocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LAMP-1/CD107a
Lysosome-Associated Membrane Protein-1 (LAMP-1), also known as CD107a, is a 100-130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP-2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP-1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28-381) and a 12 aa cytoplasmic tail (aa 405-416). The ECD has two large looping regions (aa 28-193 and 227-381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28-380, human LAMP-1 shares 64% aa identity with mouse LAMP-1.
Long Name
Lysosome-associated Membrane Glycoprotein 1
Alternate Names
CD107a, LAMP1
Gene Symbol
LAMP1
UniProt
Additional LAMP-1/CD107a Products
Product Documents for Human LAMP-1/CD107a APC-conjugated Antibody
Product Specific Notices for Human LAMP-1/CD107a APC-conjugated Antibody
For research use only
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