Chinese hamster ovary cell line CHO-derived recombinant human Integrin alphaV beta6
Phe31-Val992 (Integrin alphaV) and Gly22-Asn707 (Integrin beta6)
Accession # P18564

Specificity

Detects human Integrin beta6 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human Integrin beta6 Antibody

Detection of Integrin beta6 in HT-29 Human Cell Line by Flow Cytometry.

HT-29 human colon adenocarcinoma cell line was stained with Human Integrin beta6 Monoclonal Antibody (Catalog # MAB4155, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Integrin beta6 in HT‑29 Human Cell Line.

Integrin beta6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human Integrin beta6 Monoclonal Antibody (Catalog # MAB4155) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Integrin beta6 in HT-29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells .

Integrin beta6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line (positive) and A549 human lung carcinoma cell line (negative) cells using Mouse Anti-Human Integrin beta6 Monoclonal Antibody (Catalog # MAB4155) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Integrin beta6 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HT-29 human colon adenocarcinoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 6

Integrin beta6 (ITG beta6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alphaV and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta6 is a type I transmembrane glycoprotein that is 767 aa in length. It contains a 688 aa extracellular domain (ECD) (aa 22‑709) that incorporates a 241 aa VWF-A domain. Over aa 22‑707, human integrin beta6 ECD shares 90% and 93% aa sequence identity with mouse and pig integrin beta6 ECD, respectively.

Alternate Names

ITGB6

Entrez Gene IDs

3694 (Human); 16420 (Mouse); 311061 (Rat); 102122296 (Cynomolgus Monkey)

Gene Symbol

ITGB6

UniProt

P18564

Additional Integrin beta 6 Products

Product Documents for Human Integrin beta6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Integrin beta6 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin beta6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4155

Key Product Details

Species Reactivity

Validated:

Cited:

Applications