Detects human IL-13 R alpha2 in ELISAs. In sandwich ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) IL-4 R, rhIL-5 R alpha, rhIL‑9 R, rhIL‑13, rhIL-13 R alpha1, recombinant mouse (rm) IL-13, rmIL-13 R alpha1, or rmIL-13 R alpha2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human IL-13 R alpha2 Antibody

Human IL-13 R alpha 2 ELISA Standard Curve

Human IL-13 R alpha 2 was serially diluted and captured by Mouse Anti-Human IL-13 R alpha2 Monoclonal Antibody (Catalog # MAB6141) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-13 R alpha2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human IL-13 R alpha2 Antibody

Human IL-13 R alpha2 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human IL-13 R alpha2 Biotinylated Antibody (Catalog # BAF614)
Standard: Recombinant Human IL-13 R alpha 2 Fc Chimera (NS0), CF (Catalog # 614-INS)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB6141 in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13 R alpha 2

Two type I membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with high affinity. The lower affinity IL-13 binding protein is now referred to as IL-13 R alpha1 and is also known as CD213a. IL-13 R alpha1 combines with IL-4 R alpha to form a high affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The higher affinity IL-13 binding protein, now referred to as IL-13 R alpha2, does not induce a signal and acts as a decoy receptor (1, 2).

References

Wood, N. et al. (2003) J. Exp. Med. 197:703.
Kelly-Welch, A. et al. (2003) Science 300:1527.

Long Name

Interleukin 13 Receptor alpha 2

Alternate Names

CD213a2, IL-13Ra2, IL13R alpha 2, IL13RA2

Entrez Gene IDs

3598 (Human); 16165 (Mouse)

Gene Symbol

IL13RA2

UniProt

Q14627

Additional IL-13 R alpha 2 Products

Product Documents for Human IL-13 R alpha2 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human IL-13 R alpha2 Antibody

For research use only

Citations
Reviews

Human IL-13 R alpha2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6141

Key Product Details

Species Reactivity

Validated:

Cited:

Applications