Human GM-CSF Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM215
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
ELISA Development, Luminex Development
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # 3209
Product Specifications
Immunogen
E. coli-derived recombinant human GM-CSF
Specificity
Detects recombinant human GM-CSF in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GM-CSF Biotinylated Antibody
Human GM-CSF ELISA Standard Curve
Recombinant Human GM-CSF (Catalog # 215-GM) was serially diluted and captured by Mouse Anti-Human GM-CSF Monoclonal Antibody (Catalog # MAB615) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GM-CSF Monoclonal Antibody (Catalog # MAB215) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human GM-CSF Biotinylated Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human GM-CSF (Catalog # 215-GM)
under non-reducing conditions only
Sample: Recombinant Human GM-CSF (Catalog # 215-GM)
under non-reducing conditions only
Human GM-CSF Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using BAM215 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GM-CSF
Granulocyte macrophage colony stimulating factor (GM-CSF) is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and IL-5.
Long Name
Granulocyte Macrophage Growth Factor
Alternate Names
CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim
Entrez Gene IDs
Gene Symbol
CSF2
Additional GM-CSF Products
Product Documents for Human GM-CSF Biotinylated Antibody
Product Specific Notices for Human GM-CSF Biotinylated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...