Human GM-CSF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB615
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Intracellular Staining by Flow Cytometry, CyTOF-ready
Cited:
Western Blot, Flow Cytometry, ELISA Capture, ELISA Development, Luminex Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 6804
Product Specifications
Immunogen
E. coli-derived recombinant human GM-CSF
Ala18-Glu144
Accession # P04141
Ala18-Glu144
Accession # P04141
Specificity
Detects human GM-CSF in ELISAs. In ELISAs, no cross-reactivity with recombinant mouse GM‑CSF, recombinant rat GM‑CSF, recombinant human (rh) M‑CSF, rhG‑CSF, rhIL‑5‑R alpha, or rhIL‑3 R alpha is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GM-CSF Antibody
Detection of GM‑CSF in Human Dendritic Cells by Flow Cytometry.
Human dendritic cells treated with LPS, Recombinant Human TNF-a (210-TA), and Recombinant Human TGF-beta 1 (240-B) were stained with Mouse Anti-Human GM-CSF Monoclonal Antibody (Catalog # MAB615, filled histogram) or isotype control antibody (IC002P, open histogram) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
Detection of GM-CSF by Western Blot
Kinetics of Azithromycin Effects on NALP3 Protein in THP-1 Monocytes. a THP-1 cells were treated with azithromycin 50ug/ml for the times shown prior to immunoblot analysis for indicated proteins. b THP-1 cells were treated with LPS 500 ng/ml for times shown prior to analysis. c THP-1 cells were treated with azithromycin (50ug/ml) and LPS (500 ng/ml) for times shown prior to immunoblot analysis. The data representative of 2–3 separate experiments Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28659178), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GM-CSF by Western Blot
Kinetics of Azithromycin Effects on NALP3 Protein in THP-1 Monocytes. a THP-1 cells were treated with azithromycin 50ug/ml for the times shown prior to immunoblot analysis for indicated proteins. b THP-1 cells were treated with LPS 500 ng/ml for times shown prior to analysis. c THP-1 cells were treated with azithromycin (50ug/ml) and LPS (500 ng/ml) for times shown prior to immunoblot analysis. The data representative of 2–3 separate experiments Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28659178), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human GM-CSF Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human mature dendritic cells treated with LPS, Recombinant Human TNF‑ alpha (Catalog # 210‑TA), and Recombinant Human IL‑1 beta/IL‑1F2 (Catalog # 201-LB) fixed with paraformaldehyde and permeabilized with saponin
Sample: Human mature dendritic cells treated with LPS, Recombinant Human TNF‑ alpha (Catalog # 210‑TA), and Recombinant Human IL‑1 beta/IL‑1F2 (Catalog # 201-LB) fixed with paraformaldehyde and permeabilized with saponin
Human GM-CSF Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4 using MAB615 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GM-CSF
Long Name
Granulocyte Macrophage Growth Factor
Alternate Names
CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim
Entrez Gene IDs
Gene Symbol
CSF2
UniProt
Additional GM-CSF Products
Product Documents for Human GM-CSF Antibody
Product Specific Notices for Human GM-CSF Antibody
For research use only
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