NS0 mouse myeloma cell line transfected with human GLP-2R was stained with Mouse Anti-Human GLP-2R Monoclonal Antibody (Catalog # MAB4285, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Human GLP-2R Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: NS0 mouse myeloma cell line transfected with human GLP-2R

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLP-2R

GLP-2R is a member of the class II glucagon-secretin G protein-coupled receptor superfamily. It is expressed on enteroendocrine cells in the gut where it mediates the positive effects of GLP2 on villus height, crypt depth and intestinal energy absorbtion. After stimulating a cAMP response, lipid raft-dependent internalization of GLP‑2R results in rapid desensitization to GLP2. Extracellular portions of human GLP-2R show approximately 80% amino acid identity with corresponding regions of mouse GLP-2R.

Long Name

Glucagon-like Peptide 2 Receptor

Alternate Names

GLP2R

Entrez Gene IDs

9340 (Human); 93896 (Mouse); 60432 (Rat)

Gene Symbol

GLP2R

UniProt

O95838

Additional GLP-2R Products

Product Documents for Human GLP-2R Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GLP-2R Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GLP-2R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4285

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all GLP-2R Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GLP-2R Antibody

Detection of GLP‑2R in NS0 Mouse Cell Line Transfected with Human GLP-2R by Flow Cytometry.