Human GASP-1/WFIKKN2 Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB20701AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 288518
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human GASP‑1/WFIKKN2
Specificity
Detects human GASP‑1/WFIKKN2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human GASP-2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: GASP-1/WFIKKN2
WAP, follistatin, Kazal and netrin domains are all implicated in protease inhibition, and these proteins may be multivalent protease inhibitors (3). GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP‑1 expression is highest in the brain, skeletal muscle, thymus and kidney while GASP-2 is abundant in the lung, skeletal muscle and liver (4). In the adult, GASP-1 is primarily expressed in the ovary, testis, and brain while GASP-2 is in the pancreas, liver, and thymus (4). GASP‑1 inhibits myostatin and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (1). In addition, GASP‑1 binds directly but independently to both mature myostatin and the myostatin propeptide (1). By amino acid sequence, human GASP-1 is 90% and 55% identical to mouse GASP-1 and human GASP-2, respectively.
References
- Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
- Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. 98:3705.
- Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.
- Trexler, M. et al. (2002) Biol. Chem. 383:223.
Long Name
Growth and Differentiation Factor-associated Serum Protein-1
Alternate Names
GASP1, WFIKKN2, WFIKKNRP
Gene Symbol
WFIKKN2
UniProt
Additional GASP-1/WFIKKN2 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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