Human Fucosyltransferase 3/5 (FUT3/5) Lumenal Domain Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF4068
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fucosyltransferase 3/5 (FUT3/5) Lumenal Domain
Arg35-Thr374
Accession # Q11128
Arg35-Thr374
Accession # Q11128
Specificity
Detects Fucosyltransferase 3/5 (FUT3/5) Lumenal Domain in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT)
Recombinant Human Fucosyltransferase 5/FUT5 (Catalog # 4949-GT)
Sample: Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT)
Recombinant Human Fucosyltransferase 5/FUT5 (Catalog # 4949-GT)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fucosyltransferase 3/5 (FUT3/5)
FUT5 ( alpha-1, 3-fucosyltransferase 5; also Fuc-TV) is a 43 kDa member of the glycosyltransferase 10 family. It is responsible for the terminal step in the synthesis of Lex, sialyl‑Lex, and Lea antigens. This is accomplished by the transfer of fucose to preexisting LacNAc or sialyl-LacNAc structures. Human FUT5 is 374 amino acids (aa) in length. It is a type II transmembrane Golgi glycoprotein with a C-terminal lumenal domain (aa’s 35-374). The catalytic domain spans aa’s 77-374, while the acceptor-binding motif includes aa’s 120-126. FUT5 is the product of a single exon gene. Thus, there are no splice variants. FUT5 only occurs in primates; there is no rodent ortholog.
Alternate Names
CD174, FT3B, FucT-III, LE, Les
Entrez Gene IDs
2525 (Human)
Gene Symbol
FUT3
UniProt
Additional Fucosyltransferase 3/5 (FUT3/5) Products
Product Specific Notices
For research use only
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