Human Exosome Component 9 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11616
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1090209
Product Specifications
Immunogen
Synthetic Peptide
Accession # Q06265
Accession # Q06265
Specificity
Detects a synthetic peptide specific for hEXOSC9 around amino acid 300 in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Exosome Component 9 Antibody
Detection of Exosome Component 9 in Human Colon Cancer.
Exosome Component 9 was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human Exosome Component 9 Monoclonal Antibody (Catalog # MAB11616) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Exosome Component 9 in U251-MG Human Cell Line.
Exosome Component 9 was detected in immersion fixed U251-MG human malignant glioblastoma cell line using Mouse Anti-Human Exosome Component 9 Monoclonal Antibody (Catalog # MAB11616) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Exosome Component 9 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed U251-MG human malignant glioblastoma cell line
Sample: Immersion fixed U251-MG human malignant glioblastoma cell line
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer
Sample: Immersion fixed paraffin-embedded sections of human colon cancer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Exosome Component 9
References
- Yoshino S, Matsui Y, Fukui Y, Seki M, Yamaguchi K, Kanamori A, Saitoh Y, Shimamura T, Suzuki Y, Furukawa Y, Kaneko S, Seiki M, Murakami Y, Inoue JI, Sakamoto T. EXOSC9 depletion attenuates P-body formation, stress resistance, and tumorigenicity of cancer cells. Sci Rep. 2020 Jun 9;10(1):9275. doi: 10.1038/s41598-020-66455-2. PMID: 32518284; PMCID: PMC7283315.
- Quttina M, Waiters KD, Khan AF, Karami S, Peidl AS, Babajide MF, Pennington J, Merchant FA, Bawa-Khalfe T. Exosc9 Initiates SUMO-Dependent lncRNA TERRA Degradation to Impact Telomeric Integrity in Endocrine Therapy Insensitive Hormone Receptor-Positive Breast Cancer. Cells. 2023 Oct 20;12(20):2495. doi: 10.3390/cells12202495. PMID: 37887339; PMCID: PMC10605189.
Alternate Names
p5, p6, PCH1D, PM/Scl-75, PMSCL1, RRP45, Rrp45p
Gene Symbol
EXOSC9
UniProt
Additional Exosome Component 9 Products
Product Documents for Human Exosome Component 9 Antibody
Product Specific Notices for Human Exosome Component 9 Antibody
For research use only
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