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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 560514

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ENPP‑7/Alk‑SMase
Pro23-Ser439
Accession # Q6UWV6

Specificity

Detects human ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ENPP-2, rhENPP-5, recombinant mouse ENPP-7 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human ENPP-7/Alk-SMase Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ENPP-7/Alk-SMase

ENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is a 60 kDa GPI-linked membrane glycoprotein expressed in the intestines and in human bile. It shares 30%‑36% homology with the NPP family, but is unrelated to neutral or acid SMase. ENPP-7 hydrolyzes dietary sphingomyelin to form ceramide and phosphorylcholine, and may also hydrolyze and inactivate platelet-activating factor (PAF). It is down‑regulated in some human colorectal carcinomas. The 416 amino acid (aa) mature human ENPP-7 shares 80% and 82% aa identity with mouse and rat ENPP-7, respectively.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 7

Alternate Names

Alk-SMase, ENPP7, NPP7

Entrez Gene IDs

339221 (Human); 238011 (Mouse); 303729 (Rat)

Gene Symbol

ENPP7

UniProt

Additional ENPP-7/Alk-SMase Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human ENPP-7/Alk-SMase Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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