HEK293 human embryonic kidney cell line transfected with either (A) human EDNRA or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human EDNRA Monoclonal Antibody (Catalog # MAB65381) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Applications for Human EDNRA/Endothelin R Type A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human EDNRA and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDNRA/Endothelin R Type A

EDNRA (endothelin A receptor) is a 427 amino acid (aa) protein of the rhodopsin-like 7-transmembrane receptor family. It can be variably modified by N-linked glycoproteins, phosphorylation and palmitoylation. It binds endothelins ET-1 and ET-2 preferentially, while EDNRB binds all three forms equally. Developmentally, EDNRA is expressed by cranial neural crest cells and induces facial morphogenesis. It is expressed by adult blood vessel smooth muscle cells, and is primarily responsible for the vasoconstrictor effects of ET-1. Human EDNRA shares 86% aa identity with mouse and rat EDNRA within the combined extracellular portions of the molecule.

Long Name

Endothelin Receptor Type A

Alternate Names

EDNRA, ETAR, ETRA

Entrez Gene IDs

1909 (Human); 13617 (Mouse)

Gene Symbol

EDNRA

UniProt

NP_001948

Additional EDNRA/Endothelin R Type A Products

Product Documents for Human EDNRA/Endothelin R Type A Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human EDNRA/Endothelin R Type A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human EDNRA/Endothelin R Type A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB65381

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all EDNRA/Endothelin R Type A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human EDNRA/Endothelin R Type A Antibody

Detection of EDNRA in HEK293 Human Cell Line Transfected with Human EDNRA and eGFP by Flow Cytometry.