Human EDA/Ectodysplasin Alexa Fluor® 532-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB9222X
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair)
Label
Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 174806
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EDA-A2/Ectodysplasin A2
Ala179-Ser389
Accession # NP_001005609
Ala179-Ser389
Accession # NP_001005609
Specificity
Detects human EDA/Ectodysplasin (EDA-A1 and EDA-A2) in ELISAs. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant mouse EDA is observed, and no cross-reactivity with recombinant human (rh) TNF-alpha, rhXEDAR, or rhEDAR is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications
Application
Recommended Usage
ELISA Capture (Matched Antibody Pair)
Optimal dilution of this antibody should be experimentally determined.
ELISA Detection (Matched Antibody Pair)
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: EDA-A1/Ectodysplasin A1
The products of the Ectodysplasin A gene (EDA, also called Tabby) are type II transmembrane proteins that belong to the TNF superfamily. The EDA gene encodes two splice variants, designated EDA-A1 and EDA-A2, that differ only by the deletion of two amino acids in the extracellular domain of EDA-A2. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, a member of the TNF Receptor superfamily (TNFRSF), whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. The extracellular domains of human and mouse EDA-A2 share approximately 94% amino acid sequence identity.
Alternate Names
EDAA1
Gene Symbol
EDA
UniProt
Additional EDA-A1/Ectodysplasin A1 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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