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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Dopamine β‑Hydroxylase
Leu37-Gly617
Accession # P09172

Specificity

Detects human Dopamine beta‑Hydroxylase in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Dopamine beta-Hydroxylase

DBH (Dopamine beta-Hydroxylase) is a 77-78 kDa member of the Cu++ type II ascorbate-dependent monooxygenase family of enzymes. It is expressed in noradrenergic nerve terminals and adrenal medullary chromaffin cells, and serves as a catalyst for the conversion of dopamine into norepinepherine. Unlike its family member counterparts (TH and PNMT) that are cytosolic, DBH is embedded in the membranes of secretory vesicles. Human DBH is a 617 amino acid (aa) type II transmembrane glycoprotein (SwissProt # P09172). It contains an N-terminal 16 aa cytoplasmic region, plus a 580 aa lumenal domain (aa 38-617). The luminal region possesses one DOMON (dopamine beta-monooxygenase N-terminal) domain (aa 51-169) plus two consecutive monooxygenase motifs (aa 214-523). DBH exists as both a membrane-embedded 77-78 kDa isoform, and a soluble 73-75 kDa isoform. The latter may arise from cleavage following Gly39. Structurally, DBH will form a disulfide-linked homodimer, which then noncovalently associates with another DBH covalent homodimer, generating a functional 290 kDa homotetramer. Over aa 37‑617, human DBH shares 79% aa sequence identity with mouse DBH.

Alternate Names

DBH, DBM, Dopamine betaHydroxylase, DOPBHY

Entrez Gene IDs

1621 (Human); 13166 (Mouse); 25699 (Rat)

Gene Symbol

DBH

UniProt

Additional Dopamine beta-Hydroxylase Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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