Cytokeratin 7 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human KRT7, Monoclonal Antibody (Catalog# MAB11701) at 1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Detection of Cytokeratin 7 in human Liver via seqIF™ staining on COMET™

Cytokeratin 7 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Cytokeratin 7, Monoclonal Antibody (Catalog# MAB11701) at 1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Detection of Cytokeratin 7 in human Pancreas via seqIF™ staining on COMET™

Cytokeratin 7 was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human KRT7, Monoclonal Antibody (Catalog# MAB11701) at 1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Detection of Cytokeratin 7 in Human Kidney.

Cytokeratin 7 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Cytokeratin 7 Monoclonal Antibody (Catalog # MAB11701) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Cytokeratin 7 in Human Placenta.

Cytokeratin 7 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human Cytokeratin 7 Monoclonal Antibody (Catalog # MAB11701) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Cytokeratin 7 in SK-BR-3 cells.

Cytokeratin 7 was detected in immersion fixed SK-BR-3 human breast cancer cell line (Positive) and absent in K562 human chronic myelogenous leukemia cell line (Negative) using Rabbit Anti-Human Cytokeratin 7 Monoclonal Antibody (Catalog # MAB11701) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Cytokeratin 7 by Western Blot.

Western Blot shows lysates of HCT-116 human colorectal carcinoma cell line. PVDF membrane was probed with 0.5 µg/ml of Rabbit Anti-Human Cytokeratin 7 Monoclonal Antibody (Catalog # MAB11701) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cytokeratin 7 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human Cytokeratin 7 by Simple Western^TM.

Simple Western lane view shows lysates of KATO-III human gastric carcinoma cell line, loaded at 0.1 mg/ml. A specific band was detected for Cytokeratin 7 at approximately 56 kDa (as indicated) using 10 µg/ml of Rabbit Anti-Human Cytokeratin 7 Monoclonal Antibody (Catalog # MAB11701) followed by HRP-conjugated Goat Anti-Rabbit Secondary Antibody (Catalog # 042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

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Applications for Human Cytokeratin 7 Antibody

Application

Recommended Usage

COMET

1 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney, liver and pancreas

Immunocytochemistry

1-10 µg/mL
Sample: Immersion fixed SK-BR-3 human breast cancer cell line

Immunohistochemistry

1-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney and placenta

Simple Western

10 µg/mL
Sample: KATO-III human gastric carcinoma cell line

Western Blot

0.5 µg/mL
Sample: HCT-116 human colorectal carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytokeratin 7

Cytokeratin 7 is a 51 kDa type II keratin and a member of the keratin gene family. Cytokeratin 7 is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vesicles. It is found in simple glandular epithelia and in transitional epithelium. Combined with cytokeratin-20, cytokeratin-7 is often used for assessing the origin of metastatic cancer.

References

Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA, Wright MW. New consensus nomenclature for mammalian keratins. J Cell Biol. 2006 Jul 17;174(2):169-74. doi: 10.1083/jcb.200603161. Epub 2006 Jul 10. PMID: 16831889; PMCID: PMC2064177.
Dum D, Menz A, Völkel C, De Wispelaere N, Hinsch A, Gorbokon N, Lennartz M, Luebke AM, Hube-Magg C, Kluth M, Fraune C, Möller K, Bernreuther C, Lebok P, Clauditz TS, Jacobsen F, Sauter G, Uhlig R, Wilczak W, Steurer S, Minner S, Marx AH, Simon R, Burandt E, Krech T. Cytokeratin 7 and cytokeratin 20 expression in cancer: A tissue microarray study on 15,424 cancers. Exp Mol Pathol. 2022 Jun;126:104762. doi: 10.1016/j.yexmp.2022.104762. Epub 2022 Apr 4. PMID: 35390310.

Alternate Names

CK-7, CK7, K2C7, K7

Entrez Gene IDs

3855 (Human); 110310 (Mouse); 300242 (Rat)

Gene Symbol

KRT7

UniProt

P08729 (Human)

Additional Cytokeratin 7 Products

Product Documents for Human Cytokeratin 7 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Cytokeratin 7 Antibody

For research use only

Citations
Reviews

Human Cytokeratin 7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11701

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Product Specifications

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Scientific Data Images for Human Cytokeratin 7 Antibody

Detection of Cytokeratin 7 in human Kidneyvia seqIF™ staining on COMET™