Human Cytokeratin 7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11701
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3140B
Product Specifications
Immunogen
Synthetic Peptide
Accession # P08729
Accession # P08729
Specificity
Detects a synthetic peptide specific for human Cytokeratin 7 around amino acid 460 in Direct ELISA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Cytokeratin 7 Antibody
Detection of Cytokeratin 7 in Human Kidney.
Cytokeratin 7 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Cytokeratin 7 Monoclonal Antibody (Catalog # MAB11701) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Cytokeratin 7 in Human Placenta.
Cytokeratin 7 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human Cytokeratin 7 Monoclonal Antibody (Catalog # MAB11701) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Cytokeratin 7 in SK-BR-3 cells.
Cytokeratin 7 was detected in immersion fixed SK-BR-3 human breast cancer cell line (Positive) and absent in K562 human chronic myelogenous leukemia cell line (Negative) using Rabbit Anti-Human Cytokeratin 7 Monoclonal Antibody (Catalog # MAB11701) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Cytokeratin 7 Antibody
Application
Recommended Usage
Immunocytochemistry
1-10 µg/mL
Sample: Immersion fixed SK-BR-3 human breast cancer cell line
Sample: Immersion fixed SK-BR-3 human breast cancer cell line
Immunohistochemistry
1-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney and placenta
Sample: Immersion fixed paraffin-embedded sections of human kidney and placenta
Simple Western
10 µg/mL
Sample: KATO-III human gastric carcinoma cell line
Sample: KATO-III human gastric carcinoma cell line
Western Blot
0.5 µg/mL
Sample: HCT-116 human colorectal carcinoma cell line
Sample: HCT-116 human colorectal carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytokeratin 7
References
- Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA, Wright MW. New consensus nomenclature for mammalian keratins. J Cell Biol. 2006 Jul 17;174(2):169-74. doi: 10.1083/jcb.200603161. Epub 2006 Jul 10. PMID: 16831889; PMCID: PMC2064177.
- Dum D, Menz A, Völkel C, De Wispelaere N, Hinsch A, Gorbokon N, Lennartz M, Luebke AM, Hube-Magg C, Kluth M, Fraune C, Möller K, Bernreuther C, Lebok P, Clauditz TS, Jacobsen F, Sauter G, Uhlig R, Wilczak W, Steurer S, Minner S, Marx AH, Simon R, Burandt E, Krech T. Cytokeratin 7 and cytokeratin 20 expression in cancer: A tissue microarray study on 15,424 cancers. Exp Mol Pathol. 2022 Jun;126:104762. doi: 10.1016/j.yexmp.2022.104762. Epub 2022 Apr 4. PMID: 35390310.
Alternate Names
CK-7, CK7, K2C7, K7
Gene Symbol
KRT7
UniProt
Additional Cytokeratin 7 Products
Product Documents for Human Cytokeratin 7 Antibody
Product Specific Notices for Human Cytokeratin 7 Antibody
For research use only
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