Human Contactin-5 Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3030G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Contactin-5
Glu19-Gln1059
Accession # O94779
Glu19-Gln1059
Accession # O94779
Specificity
Detects human Contactin-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Contactin-2 and rhContactin-3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human Contactin-5 Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Contactin-5
Contactin-5 (CNTN5), also known as NB-2, is a neural adhesion molecule in the contactin family of the immunoglobulin superfamily. The contactin family comprises six members (CNTN1/F3, CNTN2/TAG-1, CNTN3/BIG-1, CNTN4/BIG-2, CNTN5/NB-2 and CNTN6/NB-3) that are characterized by the presence of six Ig-like domains, four fibronectin type III-like repeats, and a glycosylphosphatidylinositol (GPI)-anchoring domain (1, 2). The human Contactin-5 cDNA exists in two splice forms which contain an 18 amino acid (aa) signal sequence and a 28 aa C-terminal propeptide. The long isoform is a 1082 aa protein that shares 91% aa sequence identity with rat and mouse Contactin-5. The short isoform lacks the first 74 aa at the N-terminus of the long isoform. Human Contactin-5 shares 43%, 41%, 52%, 52%, and 48% aa identity with Contactins-1 ,-2 ,-3 ,-4, and -6, respectively (3). Contactin family proteins exist as membrane-bound proteins, but can also be released as soluble proteins by GPI-specific phospholipase D. The gene encoding Contactin-5 is localized to a chromosomal region associated with schizophrenia and the neuronal disorder Jacobsen syndrome (4). The highest expression of human Contactin-5 is seen in occipital lobe and amygdala, followed by cerebral cortex, frontal lobe, thalamus, and temporal lobe (4). In rat, Contactin-5 is highly expressed specifically in structures of the central auditory pathway (5). Also in rat, Contactin-5 has been shown to promote neurite outgrowth of cerebral cortical neurons in vitro (5). Deficient Contactin-5 expression in mice results in impaired neuronal activity of the central auditory system (6).
Alternate Names
CNTN5, Contactin5, NB-2
Gene Symbol
CNTN5
UniProt
Additional Contactin-5 Products
Product Specific Notices for Human Contactin-5 Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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