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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 577238

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Collagen IV α1
Ser1441-Thr1669
Accession # P02462

Specificity

Detects human Collagen IV alpha1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Collagen I alpha 1, III alpha 1, XIII alpha 1v4, XXIII alpha 1, or recombinant mouse Collagen XXV alpha 1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Collagen IV alpha 1

COL4A1 (collagen IV alpha1) is a 185 kDa member of the type IV collagen family.  It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells.  Two COL4A1 molecules interact with a 170 kDa alpha2 chain to form a collagen IV triple helix.  This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane.  Mature human COL4A1 is 1642 amino acids (aa) in length.  It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C-terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669).  Multiple splice forms exist.  One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958-1669.  Over aa 1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1.

Alternate Names

Arresten, COL4A1

Entrez Gene IDs

1282 (Human); 12826 (Mouse); 290905 (Rat)

Gene Symbol

COL4A1

UniProt

Additional Collagen IV alpha 1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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