HEK293 cells transfected with Human CLEC17A and eGFP (A) vs irrelevant cells (B) were stained with eGFP and Mouse Anti-Human CLEC17A Monoclonal Antibody (Catalog # MAB101471) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CLEC17A Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 cells transfected with Human CLEC17A and eGFP vs irrelevant

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC17A

C-type lectin domain family 17, member A (CLEC17A), also known as Prolectin, is type II transmembrane protein that is expressed mainly on dividing B cells found in the germinal centers of secondary lymphoid organs, including lymph nodes, tonsils, stomach, intestine, appendix and spleen (1, 2). CLEC17A binds preferentially to epithelial rather than to mesenchymal cells, and it behaves as a cell adhesion molecule for epithelial cells (2). It has high specificity towards mannose and was found to form disulfide-linked oligomers (1, 3). Human CLEC17A is synthesized as a 378 amino acid (aa) protein that includes a 172 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 185 aa extracellular domain (ECD). Within the ECD, human CLEC17A shares 84% aa sequence identity with canine CLEC17A. C-type lectins are Ca²⁺-depending sugar-binding proteins that are involved in several immune-related and other physiological functions. Presently, 17 groups within the C-type lectin superfamily have been recognized (4), with more C-type lectins being constantly discovered based on the presence of a conserved 115-130 amino acid domain along their sequences - the C-type carbohydrate recognition domain (CRD). However, for most of the recently identified C-type lectins, their interactions with carbohydrates, intracellular functions and molecular mechanisms still remain unclear (3).

References

Graham, S.A. et al. (2009) J. Biol. Chem. 284:18537.
Breiman, A. et al. (2016) Oncotarget 7:14064.
Koh, G. et al. (2011) BMC Bioinformatics 12:S5.
Zelensky, A.N. et al. (2005) FEBS Journal 272:6179.

Long Name

C-type Lectin Domain Family 17, Member A

Alternate Names

Prolectin

Entrez Gene IDs

388512 (Human)

Gene Symbol

CLEC17A

UniProt

Q6ZS10

Additional CLEC17A Products

Product Documents for Human CLEC17A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CLEC17A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CLEC17A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB101471

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CLEC17A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CLEC17A Antibody

Detection of CLEC17A in HEK293 cells by Flow Cytometry.