PBMC monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CLEC12B Monoclonal Antibody (Catalog # MAB11476) or (B) isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CLEC12B Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: PBMC monocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC12B

C-type lectin domain family 12 member B (CLEC12B) is a member of the C-type lectin-like family of proteins. CLEC12B is widely expressed at low levels in various human tissues except in the brain (1, 2). A truncated version lacking a portion of the carbohydrate-recognition domain (CRD) has been detected in mammary gland, lung and ovary, and was predicted to be nonfunctional (1). CLEC12B is a cell surface receptor that may play a role in viral recognition and modulate signaling cascades due to the presence of an ITIM motif within its cytoplasmic tail (1-3). Human CLEC12B is synthesized as a 276 amino acid (aa) protein that includes a 43 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 212 aa extracellular domain (ECD). Within the ECD, human CLEC12B shares 74% and 70% aa sequence identity with mouse and rat CLEC12B, respectively. The extracellular domain of CLEC12B shows considerable homology to the activating natural killer cell receptor NKG2D, and it antagonizes NKG2D mediated signaling through the ITIM motif (1). CLEC12B may be involved in limiting the activity of monocyte-derived immune cells after cell differentiation and possibly during inflammatory diseases. They play a role in HIV-1, mycobacterial, and Candida infections, and the coevolution of hosts and pathogens (4). Pathogen recognition by C-type lectins triggers signaling pathways that lead to the expression of specific cytokines which subsequently instruct adaptive T helper immune responses (4).

References

Hoffmann, S. et al. (2007) J. Biol. Chem. 282:22370.
Huysamen, C. et al. (2009) FEMS Microbiol. Lett. 290:121.
Monteiro, J.T. and B. Lepenies (2017) Viruses 9:59.
van den Berg, L.M. et al. (2012) Ann N Y Acad Sci. 1253:149.

Long Name

C-type Lectin Domain Family 12, Member B

Alternate Names

Macrophage Antigen H, UNQ5782

Entrez Gene IDs

387837 (Human); 71183 (Mouse); 502900 (Rat)

Gene Symbol

CLEC12B

UniProt

Q2HXU8

Additional CLEC12B Products

Product Documents for Human CLEC12B Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CLEC12B Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CLEC12B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11476

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CLEC12B Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CLEC12B Antibody

Detection of CLEC12B in PBMC monocytes by Flow Cytometry