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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human BRDG1
Ala177-Ser280
Accession # Q9ULZ2

Specificity

Detects endogenous human BRDG1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human BRDG1 Alexa Fluor® 594-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BRDG1

BCR downstream-signaling protein 1 (BRDG1; also known as Signal-transducing adaptor protein 1, STAP1) is a 36‑37 kDa adaptor molecule that is involved in inflammation. It is expressed in hematopoietic cells, including B cells, macrophages and microglia. BRDG1 is believed to promote and prolong the immune response by controlling cell migration, secretion and phagocytosis. It does so by likely controlling the recruitment of signaling kinases to TRKs, and potentiating BCR-mediated activation of CREB and Elk‑1. Human BRDG1 is 295 amino acids (aa) in length and contains a plextrin homology domain that binds to membrane phospholipids (aa 25‑121), and one SH2 domain that recognizes phosphorylated Tyr residues (aa 176‑259). There is a Tec-mediated phosphorylation site at Tyr65. Over amino acids 177‑280, human BRDG1 shares 82% aa identity with mouse BRDG1.

Long Name

BCR Downstream-signaling Protein 1

Alternate Names

STAP1

Entrez Gene IDs

26228 (Human); 56792 (Mouse); 305269 (Rat)

Gene Symbol

STAP1

UniProt

Additional BRDG1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human BRDG1 Alexa Fluor® 594-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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