Western blot shows lysates of human stomach tissue, human lung tissue, human liver tissue, and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6705) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Aldehyde Dehydrogenase 3-A1/ALDH3A1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 by Simple Western^TM.

Left: Simple Western lane view shows lysates of human lung, loaded at 0.1 mg/ml. A specific band was detected for Aldehyde Dehydrogenase 3-A1/ALDH3A1 at approximately 56 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Sheep Anti-Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6705) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Sheep Anti-Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6705) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the human lung.

Aldehyde Dehydrogenase 3‑A1/ALDH3A1 in A549 Human Cell Line.

Aldehyde Dehydrogenase 3-A1/ALDH3A1 was detected in immersion fixed A549 human lung carcinoma cell line using Sheep Anti-Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6705) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Applications for Human Aldehyde Dehydrogenase 3‑A1/ALDH3A1 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human normal lung and lung cancer tissue

Simple Western

10-50 µg/mL
Sample: Human lung tissue

Western Blot

0.25 µg/mL
Sample: human stomach tissue, human lung tissue, and A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aldehyde Dehydrogenase 3-A1/ALDH3A1

ALDH3A1 (Aldehyde dehydrogenase 3; also aldehyde dehydrogenase, dimeric NADP-preferring) is a 51-55 kDa, nuclear and cytosolic member of the ALDH3 family, ALDH superfamily of enzymes. It has widespread expression, being found in pancreatic acinar cells, corneal epithelium and stromal keratocytes, stem cells, and likely the epithelium of the jejunum. ALDH3A1 has many activities, including the detoxification of lipid peroxidation-derived medium chain (>5 carbon) aldehydes, the absorption of UV light in the cornea, acting as an antioxidant through the production of NADPH, and the extension of the cell cycle in expressing cells by reducing cyclin-dependent kinase activity. Notably, the critically important absorption of UVB light by ALDH3A1 in the cornea induces its aggregation and renders the molecule inactive as an enzyme. Human ALDH3A1 is 453 amino acids (aa) in length. It contains four acetylated lysines and a general catalytic region between aa 5-447. There are potentially six splice variants, one of which shows a 130 aa substitution for aa 1-247. Over aa 2-453, human ALDH3A1 shares 80% and 61% aa sequence identity with mouse ALDH3A1 asnd human ALDH3A2, respectively.

Alternate Names

Aldehyde Dehydrogenase 3A1, ALDH3A1, ALDHIII

Entrez Gene IDs

218 (Human); 11670 (Mouse); 25375 (Rat)

Gene Symbol

ALDH3A1

UniProt

P30838

Additional Aldehyde Dehydrogenase 3-A1/ALDH3A1 Products

Product Documents for Human Aldehyde Dehydrogenase 3‑A1/ALDH3A1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Aldehyde Dehydrogenase 3‑A1/ALDH3A1 Antibody

For research use only

Citations
Reviews

Human Aldehyde Dehydrogenase 3‑A1/ALDH3A1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6705

Key Product Details

Species Reactivity

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Product Specifications

Immunogen

Specificity

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Isotype

Scientific Data Images for Human Aldehyde Dehydrogenase 3‑A1/ALDH3A1 Antibody

Detection of Human Aldehyde Dehydrogenase 3‑A1/ALDH3A1 by Western Blot.

Detection of Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 by Simple WesternTM.