Glyoxalase I Antibody (266) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43618
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Predicted:
Bovine (90%), Mouse (92%), Rat (91%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 266
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (82%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse Glyoxalase I Antibody (266) - Azide and BSA Free (NBP2-43618) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Glyoxalase I Antibody (266) - Azide and BSA Free
![Immunohistochemistry-Paraffin: Glyoxalase I Antibody (266) [NBP2-43618]](https://resources.bio-techne.com/images/products/Glyoxalase-I-Antibody-266-Immunohistochemistry-Paraffin-NBP2-43618-img0004.jpg "Immunohistochemistry-Paraffin: Glyoxalase I Antibody (266) [NBP2-43618]")
Immunohistochemistry-Paraffin: Glyoxalase I Antibody (266) [NBP2-43618]
Immunohistochemistry-Paraffin: Glyoxalase I Antibody (266) [NBP2-43618] - Glyoxalase I Antibody (266) detects Glyoxalase I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer.Glyoxalase I stained by Glyoxalase I Antibody (266) diluted at 1:200.![Western Blot: Glyoxalase I Antibody (266) [NBP2-43618]](https://resources.bio-techne.com/images/products/Glyoxalase-I-Antibody-266-Western-Blot-NBP2-43618-img0001.jpg "Western Blot: Glyoxalase I Antibody (266) [NBP2-43618]")
Western Blot: Glyoxalase I Antibody (266) [NBP2-43618]
Western Blot: Glyoxalase I Antibody (266) [NBP2-43618] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract D. 30 ug A375 whole cell lysate/extract 12 % SDS-PAGE Glyoxalase I antibody [266] dilution: 1:1000.
Western Blot: Glyoxalase I Antibody (266) [NBP2-43618] -
Western Blot: Glyoxalase I Antibody (266) [NBP2-43618] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [GT266] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Applications for Glyoxalase I Antibody (266) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Glyoxalase I
Alternate Names
Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, Methylglyoxalase
Gene Symbol
GLO1
Additional Glyoxalase I Products
Product Documents for Glyoxalase I Antibody (266) - Azide and BSA Free
Product Specific Notices for Glyoxalase I Antibody (266) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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