Glucosylceramidase/GBA Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32271
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Zebrafish
Cited:
Human, Mouse, Fish - Danio rerio (Zebrafish)
Predicted:
Bovine (92%), Chimpanzee (100%), Porcine (92%), Rat (91%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Glucosylceramidase/GBA. The exact sequence is proprietary.
Reactivity Notes
Zebrafish reactivity reported in scientific literature (PMID: 25326392). Mouse reactivity reported in scientific literature (PMID: 30927072).
Localization
Lysosome membrane, Peripheral membrane protein, Lumenal side
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Glucosylceramidase/GBA Antibody - BSA Free (NBP1-32271) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Glucosylceramidase/GBA Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Glucosylceramidase/GBA Antibody - BSA Free
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-32271]](https://resources.bio-techne.com/images/products/Glucosylceramidase-GBA-Antibody-Immunohistochemistry-Paraffin-NBP1-32271-img0008.jpg "Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-32271]")
Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-32271]
Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-32271] - Lung cancer, using GBA antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.![Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271]](https://resources.bio-techne.com/images/products/Glucosylceramidase-GBA-Antibody-Western-Blot-NBP1-32271-img0013.jpg "Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271]")
Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271]
Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - Sample(30 ug of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500
Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] -
Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - MCF-7 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucosylceramidase/GBA antibody [C1C3] (NBP1-32271) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Applications for Glucosylceramidase/GBA Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Glucosylceramidase/GBA
Additional Glucosylceramidase/GBA Products
Product Documents for Glucosylceramidase/GBA Antibody - BSA Free
Product Specific Notices for Glucosylceramidase/GBA Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.