GADD45G Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84385

Novus Biologicals, part of Bio-Techne
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NBP1-84385
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GADD45 gamma Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MTLEEVRGQDTVPESTARMQGAGKALHELLLSAQRQGCLTAGVYESAKVLNVDPDNVTFCVLAAGEEDEGDIALQIHFTLIQAFCCENDIDIVRVGDVQRLAAIVG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit GADD45G Antibody - BSA Free (NBP1-84385) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-GADD45G Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GADD45G Antibody - BSA Free

Western Blot: GADD45G Antibody [NBP1-84385]

Western Blot: GADD45G Antibody [NBP1-84385]

Western Blot: GADD45G Antibody [NBP1-84385] - Analysis in control (vector only transfected HEK293T lysate) and GADD45G over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
GADD45G Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: GADD45G Antibody - BSA Free [NBP1-84385]

Immunocytochemistry/ Immunofluorescence: GADD45G Antibody - BSA Free [NBP1-84385]

Staining of human cell line SH-SY5Y shows localization to nucleus.
GADD45G Antibody - BSA Free Immunohistochemistry-Paraffin: GADD45G Antibody - BSA Free [NBP1-84385]

Immunohistochemistry-Paraffin: GADD45G Antibody - BSA Free [NBP1-84385]

Staining of human rectum shows strong cytoplasmic positivity in lymphoid cells.
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Applications for GADD45G Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GADD45 gamma

GADD45G is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta.

Long Name

Growth Arrest and DNA Damage Gene gamma

Alternate Names

GADD45g

Gene Symbol

GADD45G

Additional GADD45 gamma Products

Product Documents for GADD45G Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GADD45G Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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