FSTL5 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04852
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 21-250 of human FSTL5 (NP_064501.2). RPTKEGGYGLKSYQPLMRLRHKQEKNQESSRVKGFMIQDGPFGSCENKYCGLGRHCVTSRETGQAECACMDLCKRHYKPVCGSDGEFYENHCEVHRAACLKKQKITIVHNEDCFFKGDKCKTTEYSKMKNMLLDLQNQKYIMQENENPNGDDISRKKLLVDQMFKYFDADSNGLVDINELTQVIKQEELGKDLFDCTLYVLLKYDDFNADKHLALEEFYRAFQVIQLSLP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit FSTL5 Antibody - Azide and BSA Free (NBP3-04852) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for FSTL5 Antibody - Azide and BSA Free
![Western Blot: FSTL5 AntibodyAzide and BSA Free [NBP3-04852]](https://resources.bio-techne.com/images/products/FSTL5-Antibody-Western-Blot-NBP3-04852-img0002.jpg "Western Blot: FSTL5 AntibodyAzide and BSA Free [NBP3-04852]")
Western Blot: FSTL5 AntibodyAzide and BSA Free [NBP3-04852]
Western Blot: FSTL5 Antibody [NBP3-04852] - Analysis of extracts of various cell lines, using FSTL5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
Immunocytochemistry/ Immunofluorescence: FSTL5 Antibody - Azide and BSA Free [NBP3-04852] -
Immunocytochemistry/ Immunofluorescence: FSTL5 Antibody - Azide and BSA Free [NBP3-04852] - Immunofluorescence analysis of paraffin-embedded mouse brain using FSTL5 Rabbit pAb (A16553) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for FSTL5 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Follistatin-like 5/FSTL5
Follistatin-like 5 (FSTL5) is a secreted SPARC family protein that is expressed in the cartilage and tendon of developing digits and the shafts of long bones. In the brain it is expressed in the olfactory bulb, CA3 area of the hippocampus, and granule cell layer of the cerebellum. FSTL5 inhibits the proliferation and promotes the apoptosis of hepatocellular carcinoma cells. Its expression in medulloblastoma and downregulation in hepatocellular carcinoma are associated with poor prognosis.
Alternate Names
Follistatin like 5, FSL5, FSTL5
Gene Symbol
FSTL5
Additional Follistatin-like 5/FSTL5 Products
Product Documents for FSTL5 Antibody - Azide and BSA Free
Product Specific Notices for FSTL5 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov