FGFR1 [p Tyr653, p Tyr654] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13251
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Chicken (100%), Fish (100%), Rhesus Macaque (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr653/Tyr654 of human FGFR1. The exact sequence is proprietary.
Modification
p Tyr653, p Tyr654
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for FGFR1 [p Tyr653, p Tyr654] Antibody
![Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]](https://resources.bio-techne.com/images/products/FGFR1-[p-Tyr653--p-Tyr654]-Antibody-Western-Blot-NBP3-13251-img0003.jpg "Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]")
Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]
Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]](https://resources.bio-techne.com/images/products/FGFR1-[p-Tyr653--p-Tyr654]-Antibody-Western-Blot-NBP3-13251-img0001.jpg "Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]")
Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]
Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Untreated (-) and treated (+) U87-MG whole cell extracts (40 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]](https://resources.bio-techne.com/images/products/FGFR1-[p-Tyr653--p-Tyr654]-Antibody-Western-Blot-NBP3-13251-img0002.jpg "Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]")
Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]
Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for FGFR1 [p Tyr653, p Tyr654] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry-Frozen
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FGFR1
FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.
Long Name
Fibroblast Growth Factor Receptor 1
Alternate Names
CD331, FGF R1, Flt-2
Gene Symbol
FGFR1
Additional FGFR1 Products
Product Documents for FGFR1 [p Tyr653, p Tyr654] Antibody
Product Specific Notices for FGFR1 [p Tyr653, p Tyr654] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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