FEN-1 Antibody (3H9L9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16730
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3H9L9 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human FEN-1 (P39748). IQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit FEN-1 Antibody (3H9L9) (NBP3-16730) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for FEN-1 Antibody (3H9L9)
![Western Blot: FEN-1 Antibody (3H9L9) [NBP3-16730]](https://resources.bio-techne.com/images/products/FEN-1-Antibody-3H9L9-Western-Blot-NBP3-16730-img0002.jpg "Western Blot: FEN-1 Antibody (3H9L9) [NBP3-16730]")
Western Blot: FEN-1 Antibody (3H9L9) [NBP3-16730]
Western Blot: FEN-1 Antibody (3H9L9) [NBP3-16730] - Western blot analysis of extracts of various cell lines, using FEN-1 Rabbit mAb (NBP3-16730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunohistochemistry-Paraffin: FEN-1 Antibody (3H9L9) [NBP3-16730]](https://resources.bio-techne.com/images/products/FEN-1-Antibody-3H9L9-Immunohistochemistry-Paraffin-NBP3-16730-img0001.jpg "Immunohistochemistry-Paraffin: FEN-1 Antibody (3H9L9) [NBP3-16730]")
Immunohistochemistry-Paraffin: FEN-1 Antibody (3H9L9) [NBP3-16730]
Immunohistochemistry-Paraffin: FEN-1 Antibody (3H9L9) [NBP3-16730] - Immunohistochemistry of paraffin-embedded human colon carcinoma using FEN-1 Rabbit mAb (NBP3-16730) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for FEN-1 Antibody (3H9L9)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FEN-1
The FEN-1 enzyme is active in base excision repair (BER) pathways. Once the DNA glycosylase removes the desired nucleotide stretch, a DNA polymerase synthesizes the complementary nucleotides for replacement. To complete the DNA repair, the FEN-1 enzyme removes the extra flap of nucleotides and DNA ligase finishes the repair.
Long Name
Flap Endonuclease 1
Alternate Names
FEN1
Gene Symbol
FEN1
Additional FEN-1 Products
Product Documents for FEN-1 Antibody (3H9L9)
Product Specific Notices for FEN-1 Antibody (3H9L9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...