Exosome Component 9 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71702

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Chicken

Cited:

Human, Mouse, Avian - Chicken

Predicted:

Bovine (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Exosome Component 9 Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to an internal region of human Exosome Component 9 (within residues 250-439). [Swiss-Prot Q06265]

Reactivity Notes

Immunogen has 83% identity to rat and 90% identity to bovine.

Localization

Cytoplasm. Nucleus - nucleolus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Exosome Component 9 Antibody - BSA Free (NBP1-71702) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, Simple Western, IP and ChIP. Anti-Exosome Component 9 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Exosome Component 9 Antibody - BSA Free

Western Blot: Exosome Component 9 AntibodyBSA Free [NBP1-71702]

Western Blot: Exosome Component 9 AntibodyBSA Free [NBP1-71702]

Western Blot: Exosome Component 9 Antibody [NBP1-71702] - Analysis of EXOSC9 in A. HepG2 cell lysate and B. MCF7 cell lysate.
Immunocytochemistry/ Immunofluorescence: Exosome Component 9 Antibody - BSA Free [NBP1-71702]

Immunocytochemistry/ Immunofluorescence: Exosome Component 9 Antibody - BSA Free [NBP1-71702]

Immunocytochemistry/Immunofluorescence: Exosome Component 9 Antibody [NBP1-71702] - EXOSC9 antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: Exosome Component 9 Antibody - BSA Free [NBP1-71702]

Immunohistochemistry: Exosome Component 9 Antibody - BSA Free [NBP1-71702]

Immunohistochemistry: Exosome Component 9 Antibody [NBP1-71702] - Staining of EXOSC9 in mouse prostate.
View 1 more images

Applications for Exosome Component 9 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 27543448)

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Immunoprecipitation

reported by customer review

Simple Western

1:1000

Western Blot

1:5000
Application Notes
In Western blot, a band is seen ~75 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:1000, apparent MW was 62 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-71702 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Exosome Component 9

Exosome component 9 is part of the 3' -> 5' exoribonuclease complex that helps to degrade unstable mRNA. It binds to ARE-containing RNAs and performs 3' end trimming and degradation. These activities lead toward the maturation of RNA into more stable forms, which then become involved in transcription and translation. In the related Arabidopsis equivalent, loss of function of this protein is a lethal mutation. In ruminants, EXOSC9 is differentially expressed throughout the gut at different stages of development. EXOSC9 seems to be important in the degradation of histone mRNA and may be important in some downstream epigenetic effects.

Alternate Names

p5, p6, PCH1D, PM/Scl-75, PMSCL1, RRP45, Rrp45p

Entrez Gene IDs

5393 (Human); 50911 (Mouse)

Gene Symbol

EXOSC9

UniProt

Q06265 (Human), Q9JHI7 (Mouse)

Additional Exosome Component 9 Products

Product Documents for Exosome Component 9 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Exosome Component 9 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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