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E. Coli O157 Antibody (9.88) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54353

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

E. coli

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # 9.88

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

A crude sonicate of E. coli O157

Reactivity Notes

E. coli O157

Localization

Whole organism

Specificity

This monoclonal antibody shows specificity to E.coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). TheE. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenicE. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenicE. coli(EPEC), enteroinvasiveE. coli(EIEC), enterotoxigenicE. coli(ETEC) and enterohemorrhagicE. coli(EHEC). Although the identification of pathogenicE. coli requires verification of their pathogenicity, pathogenicE. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenicE. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53146).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for E. Coli O157 Antibody (9.88) - Azide and BSA Free

Application
Recommended Usage

ELISA

1 - 5 ug/ml

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml
Application Notes
ELISA: For coating use Ab at 1-5ug/ml, order Ab without BSA.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein L or PEG purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: E. Coli O157

Alternate Names

H7 ompC

Additional E. Coli O157 Products

Product Documents for E. Coli O157 Antibody (9.88) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for E. Coli O157 Antibody (9.88) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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