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E. Coli O157 Antibody (9.88)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53146

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-53146-20ug
NBP2-53146-100ug

Key Product Details

Species Reactivity

Validated:

E. coli

Cited:

Bacteria

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

In-vitro

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # 9.88

Concentration

0.2 mg/ml

Product Specifications

Immunogen

A crude sonicate of E. coli O157

Reactivity Notes

E. coli O157

Localization

Whole organism

Specificity

This monoclonal antibody shows specificity to E.coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). TheE. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenicE. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenicE. coli(EPEC), enteroinvasiveE. coli(EIEC), enterotoxigenicE. coli(ETEC) and enterohemorrhagicE. coli(EHEC). Although the identification of pathogenicE. coli requires verification of their pathogenicity, pathogenicE. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenicE. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54353)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for E. Coli O157 Antibody (9.88)

Application
Recommended Usage

ELISA

1-5 ug/ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
ELISA: For coating use Ab at 1-5ug/ml, order Ab without BSA.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein L or PEG purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: E. Coli O157

Alternate Names

H7 ompC

Additional E. Coli O157 Products

Product Documents for E. Coli O157 Antibody (9.88)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for E. Coli O157 Antibody (9.88)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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