Dysferlin Antibody (3U1P9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15781
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3U1P9 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Dysferlin (O75923). FEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
237 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Dysferlin Antibody (3U1P9) (NBP3-15781) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Dysferlin Antibody (3U1P9)
![Western Blot: Dysferlin Antibody (3U1P9) [NBP3-15781]](https://resources.bio-techne.com/images/products/Dysferlin-Antibody-3U1P9-Western-Blot-NBP3-15781-img0002.jpg "Western Blot: Dysferlin Antibody (3U1P9) [NBP3-15781]")
Western Blot: Dysferlin Antibody (3U1P9) [NBP3-15781]
Western Blot: Dysferlin Antibody (3U1P9) [NBP3-15781] - Western blot analysis of extracts of RD cells, using Dysferlin Rabbit mAb (NBP3-15781) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry: Dysferlin Antibody (3U1P9) [Dysferlin] -
Immunohistochemistry: Dysferlin Antibody (3U1P9) [Dysferlin] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Dysferlin Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry: Dysferlin Antibody (3U1P9) [NBP3-15781] -
Immunohistochemistry: Dysferlin Antibody (3U1P9) [NBP3-15781] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Dysferlin Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Applications for Dysferlin Antibody (3U1P9)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL.
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Dysferlin
Alternate Names
dysferlin, dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive), dystrophy-associated fer-1-like 1, FER1L1Dystrophy-associated fer-1-like protein, Fer-1-like protein 1, FLJ00175, FLJ90168, LGMD2B
Gene Symbol
DYSF
Additional Dysferlin Products
Product Documents for Dysferlin Antibody (3U1P9)
Product Specific Notices for Dysferlin Antibody (3U1P9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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