Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # H00001158-M06

Novus Biologicals, part of Bio-Techne
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H00001158-M06
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F3-B11

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Creatine Kinase, Muscle/CKMM Antibodies »

Product Specifications

Immunogen

CKM (AAH07462, 1 a.a. ~ 381 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPFGNTHNKFKLNYKPEEEYPDLSKHNNHMAKVLTLELYKKLRDKETPSGFTVDDVIQTGVDNPGHPFIMTVGCVAGDEESYEVFKELFDPIISDCHGGYKPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGYTLPPHCSRGERRAVEKLSVEALNSLAGEFKGKYYPLKSMTEKEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKSLLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNQHLGYVLTCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK

Specificity

CKM - creatine kinase, muscle (2F3-B11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) - Azide and BSA Free

Western Blot: Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) [H00001158-M06]

Western Blot: Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) [H00001158-M06]

Western Blot: Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) [H00001158-M06] - Analysis of CKM expression in transfected 293T cell line by CKM monoclonal antibody (M06), clone 2F3-B11. Lane 1: CKM transfected lysate (Predicted MW: 43.1 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) [H00001158-M06]

Immunocytochemistry/ Immunofluorescence: Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) [H00001158-M06]

Immunocytochemistry/Immunofluorescence: Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) [H00001158-M06] - Analysis of monoclonal antibody to CKM on HeLa cell. Antibody concentration 40 ug/ml
Western Blot: Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) [H00001158-M06]

Western Blot: Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) [H00001158-M06]

Western Blot: Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) [H00001158-M06] - Analysis of CKM transfected lysate using anti-CKM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CKM rabbit polyclonal antibody.

Applications for Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Creatine Kinase, Muscle/CKMM

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq]

Long Name

Creatine Kinase, Muscle

Alternate Names

CKM, CKMM, CKMMcreatine kinase M-type, Creatine Kinase Isoenzyme, Creatine kinase M chain, creatine kinase, muscle, creatine kinase-M, EC 2.7.3, EC 2.7.3.2, M-CK

Entrez Gene IDs

1158 (Human)

Gene Symbol

CKM

UniProt

AAH07462 (Human)

Additional Creatine Kinase, Muscle/CKMM Products

Product Documents for Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Creatine Kinase, Muscle/CKMM Antibody (2F3-B11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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