Cotton Rat IFN-gamma Antibody

R&D Systems, part of Bio-Techne | Catalog # AF565

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AF565-SP
AF565
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Key Product Details

Species Reactivity

Validated:

Cotton Rat

Cited:

Mouse, Cotton Rat

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Flow Cytometry, Immunoprecipitation, ELISA Development, ELISA Development (Capture), ELISpot Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all IFN-gamma Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant cotton rat IFN-gamma
His21-Ile170
Accession # Q9QXX2

Specificity

Detects cotton rat IFN‑ gamma in ELISAs and Western blots. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant canine IFN-gamma, recombinant feline IFN-gamma, and recombinant human IFN-gamma, recombinant mouse IFN-gamma, recombinant rat IFN-gamma, and recombinant porcine IFN-gamma is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Cotton Rat IFN-gamma Antibody

IFN‑ gamma Inhibition of EMC-induced Cytopathy and Neutralization by Cotton Rat IFN‑ gamma Antibody.

IFN‑ gamma Inhibition of EMC-induced Cytopathy and Neutralization by Cotton Rat IFN‑ gamma Antibody.

Recombinant Cotton Rat IFN-gamma (Catalog # 565-RG) reduces the encephalomyocarditis (EMC)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMC activity elicited by Recombinant Cotton Rat IFN-gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565). The ND50 is typically 0.3-1.2 µg/mL.
Cotton Rat IFN-gamma ELISA Standard Curve

Cotton Rat IFN-gamma ELISA Standard Curve

Recombinant Cotton Rat IFN-gamma (Catalog # 565-RG) was serially diluted and captured by Goat Anti-Cotton Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Cotton Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Cotton Rat IFN-gamma ELISA Standard Curve

Cotton Rat IFN-gamma ELISA Standard Curve

Recombinant Cotton Rat IFN-gamma (Catalog # 565-RG) was serially diluted and captured by Goat Anti-Cotton Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Cotton Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Cotton Rat IFN-gamma Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Cotton Rat IFN-gamma (Catalog # 565-RG)

Neutralization

Measured by its ability to neutralize IFN‑ gamma inhibition of EMC-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 20 ng/mL Recombinant Cotton Rat IFN‑ gamma.

Cotton Rat IFN-gamma Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Cotton Rat IFN-gamma Biotinylated Antibody (Catalog # BAF565)
  • Standard: Recombinant Cotton Rat IFN-gamma Protein (Catalog # 565-RG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature cotton rat IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 43%‑50% amino acid sequence identity with bovine, canine, equine, feline, human, mouse, rat, rhesus, and porcine IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References

  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Houard, S. et al. (1999) Gene 240:107.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Gene Symbol

IFNG

UniProt

Q9QXX2

Additional IFN-gamma Products

Product Documents for Cotton Rat IFN-gamma Antibody

Product Datasheets

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Cotton Rat IFN-gamma Antibody

For research use only

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