CD117/c-kit Antibody (104D2)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-765

Discontinued Product
NB600-765 has been discontinued. View all CD117/c-kit products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Monkey

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 104D2

Concentration

0.5 mg/ml

View all CD117/c-kit Antibodies »

Product Specifications

Immunogen

Unfortunately, this information is considered to be commercially sensitive

Reactivity Notes

Cross reacts with Cynomolgus Monkey. Mouse reactivity reported in scientific literature (PMID: 24664884) Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Type I membrane protein.

Specificity

This recognises the human c-Kit. c-Kit is expressed by some haematopoietic stem cells (approx. 70% of CD34 +ve cells in normal bone marrow are c-Kit +ve), by some acute myeloid leukaemias and by mast cells.This is non-neutralising.

Marker

Hematopoietic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for CD117/c-kit Antibody (104D2)

Application
Recommended Usage

Flow Cytometry

1:2-1:20
Application Notes
Flow Cytometry Use at a dilution of 1/2 - 1/20. Use 10ul of the suggested working dilution to label 106 cells in 100ul.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24664884)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Phosphate buffered saline

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD117/c-kit

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

Additional CD117/c-kit Products

Product Documents for CD117/c-kit Antibody (104D2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD117/c-kit Antibody (104D2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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