CCDC5 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49164
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human CCDC5 fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit CCDC5 Antibody - Azide and BSA Free () is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CCDC5 Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CCDC5 Antibody - Azide and BSA Free [NBP3-49164] -
Immunocytochemistry/ Immunofluorescence: CCDC5 Antibody - Azide and BSA Free [NBP3-49164] - Immunofluorescence staining of CCDC5 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-49164 (dilution ratio 1:200) at 4C overnight. Then cells were stained with Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
Western Blot: CCDC5 Antibody - Azide and BSA Free [NBP3-49164] -
Western Blot: CCDC5 Antibody - Azide and BSA Free [NBP3-49164] - Lane A: HL60 Whole Cell Lysate. Lane B: U937 Whole Cell Lysate. Lane C: MOLT4 Whole Cell Lysate. Lane D: Daudi Whole Cell Lysate Lysates/proteins at 30 ug per lane using NBP3-49164 at 1:500 dilution. Secondary: HRP-conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDaApplications for CCDC5 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS (pH 7.0)
Format
Azide and BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CCDC5
Alternate Names
CCDC5Enhancer of invasion-cluster, coiled-coil domain containing 5 (spindle associated), FLJ40084, HAUS augmin-like complex subunit 1, HAUS augmin-like complex, subunit 1, HEI-CCoiled-coil domain-containing protein 5, HEICFLJ21094, HsT1461
Gene Symbol
HAUS1
Additional CCDC5 Products
Product Documents for CCDC5 Antibody - Azide and BSA Free
Product Specific Notices for CCDC5 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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