Cathepsin X/Z/P Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38614
![Simple Western: Cathepsin X/Z/P Antibody [NBP2-38614]](https://resources.bio-techne.com/images/products/Cathepsin-Z-Antibody-Simple-Western-NBP2-38614-img0003.jpg "Simple Western: Cathepsin X/Z/P Antibody [NBP2-38614]")
![Simple Western: Cathepsin X/Z/P Antibody [NBP2-38614]](https://resources.bio-techne.com/images/products/Cathepsin-Z-Antibody-Simple-Western-NBP2-38614-img0004.jpg "Simple Western: Cathepsin X/Z/P Antibody [NBP2-38614]")
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (91%), Rat (92%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PDETCNNYQAKDQECDKFNQCGTCNEFKECHAIRNYTLWRVGDYGSLSGREKMMAEIYANGPISCGIMATERLANYTGGIYAEYQD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Cathepsin X/Z/P Antibody - BSA Free (NBP2-38614) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Cathepsin X/Z/P Antibody - BSA Free
![Western Blot: Cathepsin X/Z/P Antibody [NBP2-38614]](https://resources.bio-techne.com/images/products/Cathepsin-Z-Antibody-Western-Blot-NBP2-38614-img0005.jpg "Western Blot: Cathepsin X/Z/P Antibody [NBP2-38614]")
Western Blot: Cathepsin X/Z/P Antibody [NBP2-38614]
Western Blot: Cathepsin X/Z/P Antibody [NBP2-38614] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line CACO-2![Immunocytochemistry/ Immunofluorescence: Cathepsin X/Z/P Antibody [NBP2-38614]](https://resources.bio-techne.com/images/products/Cathepsin-Z-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38614-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Cathepsin X/Z/P Antibody [NBP2-38614]")
Immunocytochemistry/ Immunofluorescence: Cathepsin X/Z/P Antibody [NBP2-38614]
Immunocytochemistry/Immunofluorescence: Cathepsin X/Z/P Antibody [NBP2-38614] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: Cathepsin X/Z/P Antibody [NBP2-38614]](https://resources.bio-techne.com/images/products/Cathepsin-Z-Antibody-Immunohistochemistry-Paraffin-NBP2-38614-img0007.jpg "Immunohistochemistry-Paraffin: Cathepsin X/Z/P Antibody [NBP2-38614]")
Immunohistochemistry-Paraffin: Cathepsin X/Z/P Antibody [NBP2-38614]
Immunohistochemistry-Paraffin: Cathepsin X/Z/P Antibody [NBP2-38614] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.Applications for Cathepsin X/Z/P Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Simple Western
1:10
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in SW480 lysates, separated by Size, antibody dilution of 1:10
See Simple Western Antibody Database for Simple Western validation: Tested in SW480 lysates, separated by Size, antibody dilution of 1:10
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cathepsin X/Z/P
Cathepsin X, also known as Cathepsin Z and Cathepsin P, is a relatively new cysteine protease of the papain family. Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue.
Additional Cathepsin X/Z/P Products
Product Documents for Cathepsin X/Z/P Antibody - BSA Free
Product Specific Notices for Cathepsin X/Z/P Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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