Calbindin D-28K Antibody (rCALB1/13280)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44623
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rCALB1/13280
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 7-96) of human Calbindin 1/2 protein (Uniprot: P05937). Exact sequence is proprietary.
Localization
Nucleus. Cytoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of Ab produced in CHO cell mammalian-based expression system.. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Applications for Calbindin D-28K Antibody (rCALB1/13280)
Application
Recommended Usage
Immunohistochemistry
1-2ug/ml
Western Blot
2-4ug/ml
Application Notes
Immunohistochemistry: 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Calbindin D-28K
Alternate Names
CAB27, CALB, calbindin, calbindin 1, (28kD), calbindin 1, 28kDa, Calbindin D28, D-28K, RTVL-H protein, Vitamin D-dependent calcium-binding protein, avian-type
Additional Calbindin D-28K Products
Product Documents for Calbindin D-28K Antibody (rCALB1/13280)
Product Specific Notices for Calbindin D-28K Antibody (rCALB1/13280)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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