Bmf Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76666

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

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Product Specifications

Immunogen

Antibody was raised with a synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human Bmf. The immunogen is located within the last 50 amino acids of Bmf. Amino Acid Squence: ALNGEENRNGAGPR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Bmf Antibody - BSA Free (NBP1-76666) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Bmf Antibody - BSA Free

Western Blot: Bmf AntibodyBSA Free [NBP1-76666]

Western Blot: Bmf AntibodyBSA Free [NBP1-76666]

Western Blot: Bmf Antibody [NBP1-76666] - Western blot analysis of Bmf expression in HepG2 cell lysate with Bmf antibody at (A) 2.5 and (B) 5 ug/mL.
Bmf Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Bmf Antibody - BSA Free [NBP1-76666] -

Immunocytochemistry/ Immunofluorescence: Bmf Antibody - BSA Free [NBP1-76666] - Immunofluorescence of Bmf in Human Kidney cells with Bmf antibody at 20 ug/mL.

Applications for Bmf Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

10 ug/mL

Western Blot

2.5-5 ug/mL
Application Notes
A band at approximately 25 kDa can be detected in Western Blot.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BMF

Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.

Long Name

Bcl-2 Modifying Factor

Alternate Names

Bcl2 modifying factor, bcl-2-modifying factor, FLJ00065

Gene Symbol

BMF

UniProt

NP_277038 (Human)

Additional BMF Products

Product Documents for Bmf Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bmf Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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