ASPA Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31754
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Monkey
Predicted:
Bovine (93%), Porcine (95%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human ASPA. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%).
Localization
Cytoplasm, Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit ASPA Antibody - BSA Free (NBP1-31754) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ASPA Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: ASPA Antibody [NBP1-31754]](https://resources.bio-techne.com/images/products/ASPA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31754-img0008.jpg "Immunocytochemistry/ Immunofluorescence: ASPA Antibody [NBP1-31754]")
Immunocytochemistry/ Immunofluorescence: ASPA Antibody [NBP1-31754]
Immunocytochemistry/Immunofluorescence: ASPA Antibody [NBP1-31754] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aspartoacylase protein stained by partoacylase antibody [N1C3-2] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.![Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-31754]](https://resources.bio-techne.com/images/products/ASPA-Antibody-Immunohistochemistry-Paraffin-NBP1-31754-img0003.jpg "Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-31754]")
Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-31754]
Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-31754] - Cal27 xenograft, using Aspartoacylase antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.![Western Blot: ASPA Antibody [NBP1-31754]](https://resources.bio-techne.com/images/products/ASPA-Antibody-Western-Blot-NBP1-31754-img0011.jpg "Western Blot: ASPA Antibody [NBP1-31754]")
Western Blot: ASPA Antibody [NBP1-31754]
Western Blot: ASPA Antibody [NBP1-31754] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Aspartoacylase antibody [N1C3-2] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for ASPA Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ASPA
Alternate Names
ACY-2, ACY2aminoacylase 2, Aminoacylase-2, ASPaminoacylase-2, aspartoacylase, aspartoacylase (aminoacylase 2, Canavan disease), EC 3.5.1.15
Gene Symbol
ASPA
Additional ASPA Products
Product Documents for ASPA Antibody - BSA Free
Product Specific Notices for ASPA Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.