ARF4 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92502
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ARF4 (NP_001651.1). MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDRIRPLWKHYFQNTQGLIFVVDSNDRERIQEVADELQKMLLVDELRDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit ARF4 Antibody - BSA Free (NBP2-92502) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ARF4 Antibody - BSA Free
![Western Blot: ARF4 AntibodyAzide and BSA Free [NBP2-92502]](https://resources.bio-techne.com/images/products/ARF4-Antibody-Western-Blot-NBP2-92502-img0001.jpg "Western Blot: ARF4 AntibodyAzide and BSA Free [NBP2-92502]")
Western Blot: ARF4 AntibodyAzide and BSA Free [NBP2-92502]
Western Blot: ARF4 Antibody [NBP2-92502] - Analysis of extracts of various cell lines, using ARF4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: ARF4 Antibody - Azide and BSA Free [NBP2-92502] -
Immunocytochemistry/ Immunofluorescence: ARF4 Antibody - Azide and BSA Free [NBP2-92502] - Immunofluorescence analysis of C6 cells using ARF4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: ARF4 Antibody - Azide and BSA Free [NBP2-92502] -
Immunocytochemistry/ Immunofluorescence: ARF4 Antibody - Azide and BSA Free [NBP2-92502] - Immunofluorescence analysis of NIH/3T3 cells using ARF4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for ARF4 Antibody - BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ARF4
Alternate Names
ADP-ribosylation factor 4, ARF2ADP-ribosylation factor 2
Gene Symbol
ARF4
Additional ARF4 Products
Product Documents for ARF4 Antibody - BSA Free
Product Specific Notices for ARF4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov