alpha Tubulin Antibody (DM1AR) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38646
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Chicken, Chinese Hamster, Drosophila, Fungi, Goat, Guinea Pig, Hamster, Monkey, Parasite, Primate, Rabbit, Xenopus, Yeast
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # DM1AR expressed in HEK293
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This Recombinant alpha Tubulin Antibody (DM1AR) was developed against native chicken brain microtubules.
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:34871568) Use in Mouse reported in scientific literature (PMID:34533563). Yeast reactivity reported in scientific literature (PMID: 25126732). Goat reactivity reported in scientific literature (PMID:31805146). Will likely react with all mammals.
Marker
Microtubule Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Novus Biologicals Mouse alpha Tubulin Antibody (DM1AR) - BSA Free (NB100-690) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for alpha Tubulin Antibody (DM1AR) - BSA Free
Detection of alpha Tubulin (DM1AR) in U-251 MG Human Cell Line by Flow Cytometry.
An intracellular stain was performed on immersion fixed U-251 MG human glioblastoma cell line with Mouse anti-alpha Tubulin (DM1AR) Protein-G purified Recombinant Monoclonal Antibody (Catalog # NBP3-38646, blue histogram) or matched control antibody (Catalog # MAB002, orange histogram). Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature.
alpha Tubulin (DM1AR) in U-2 OS Human Cell Line.
alpha Tubulin (DM1AR) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-alpha Tubulin (DM1AR) Protein G-purified Recombinant Monoclonal Antibody (Catalog # NBP3-38646) at 1.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Mouse IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
alpha Tubulin (DM1AR) in NIH-3T3 Mouse Cell Line.
alpha Tubulin (DM1AR) was detected in immersion fixed NIH3T3 mouse fibroblast cell line using Mouse anti-alpha Tubulin (DM1AR) Protein G-purified Recombinant Monoclonal Antibody (Catalog # NBP3-38646) at 1.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Mouse IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.Applications for alpha Tubulin Antibody (DM1AR) - BSA Free
Application
Recommended Usage
Flow (Intracellular)
1 ug per million cells
Flow Cytometry
1 ug per million cells
Immunocytochemistry/ Immunofluorescence
1 ug/ml
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Western Blot
1:5000
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: alpha Tubulin
Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).
References
1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921
2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718
Long Name
Tubulin Alpha 1a
Alternate Names
Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1, alpha tubulin loading control, alpha tubulin monoclonal, DM1A, DM1A anti-tubulin, dm1a monoclonal, DM1a tubulin, TUBA1 monoclonal, tubulin monoclonal
Gene Symbol
TUBA1A
Additional alpha Tubulin Products
Product Documents for alpha Tubulin Antibody (DM1AR) - BSA Free
Product Specific Notices for alpha Tubulin Antibody (DM1AR) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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