Acetyl Histone H3 Antibody Pack
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20192
Key Product Details
Species
Human, Mouse, Rat, C. elegans
Applications
Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Product Summary for Acetyl Histone H3 Antibody Pack
This pack contains 1 vial each of: NBP2-36468SS (0.025 mg), NB21-1074SS (0.025 mg), NB21-1024SS (0.025 mg), NB21-1144SS (0.025 mg), NB21-1164SS (0.025 mg), NB21-1254SS (0.025 mg), HAF007 (1.0 ml) and HAF008 (1.0 ml).
Product Specifications
Application Notes
See individual datasheets of components for their validated applications
Reactivity Notes
See individual datasheets of components for their validated species
Scientific Data Images for Acetyl Histone H3 Antibody Pack
Detection of Histone H3 by Immunohistochemistry-Paraffin.
Histone H3 was detected in formalin fixed paraffin-embedded sections of human breast cancer using Anti-Human Histone H3 Monoclonal Antibody (Catalog # NBP2-36468) at 1:200 overnight at 4C. The staining was developed using an HRP labeled secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Detection of Acetyl Histone H3 in Histone H3 peptides by Dot Blot.
Analysis of Histone H3 K9 acetyl with in picomoles of peptide with NB21-1074.
Detection of Histone H3 [ac Lys4] in HeLa by Chromatin Immunoprecipitation.
Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1024 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.Kit Contents for Acetyl Histone H3 Antibody Pack
- HAF007: Goat anti-Mouse IgG Secondary Antibody [HRP]
- HAF008: Goat anti-Rabbit IgG Secondary Antibody [HRP]
- NB21-1024SS
- NB21-1074SS
- NB21-1144SS
- NB21-1164SS
- NB21-1254SS
- NBP2-36468SS
Formulation, Preparation, and Storage
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
Storage
Storage is content dependent.
Product Documents for Acetyl Histone H3 Antibody Pack
Product Specific Notices for Acetyl Histone H3 Antibody Pack
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.
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