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ABH1 Antibody (23GB1265)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50950

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NBP3-50950-100ul
NBP3-50950-20ul

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1265

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human ABH1 (Uniprot: Q13686)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ABH1 Antibody (23GB1265)

ABH1 Antibody (23GB1265)

Immunocytochemistry/ Immunofluorescence: ABH1 Antibody (23GB1265)[NBP3-50950] -

Immunocytochemical staining of HepG2 cells with ABH1 antibody (1:1,000). Nuclei were stained blue with DAPI; ABH1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
ABH1 Antibody (23GB1265)

Flow Cytometry: ABH1 Antibody (23GB1265)[NBP3-50950] -

Flow cytometric analysis of ABH1expression in HepG2 cells using ABH1 antibody (1:2,000). Green, isotype control; red, ALKBH1.
ABH1 Antibody (23GB1265)

Western Blot: ABH1 Antibody (23GB1265)[NBP3-50950] -

Western blotting analysis using ABH1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for ABH1 Antibody (23GB1265)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ABH1

ABH1 encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine.

Alternate Names

ABH1, ABHalkB, alkylation repair homolog (E. coli), alkB, alkB, alkylation repair homolog 1 (E. coli), ALKBH, alkylated DNA repair protein alkB homolog 1, alkylation repair, alkB homolog, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA lyase ABH1, EC 1.14.11.-, EC 4.2.99.18, hABH

Gene Symbol

ALKBH1

Additional ABH1 Products

Product Documents for ABH1 Antibody (23GB1265)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ABH1 Antibody (23GB1265)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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