8-oxo-dG Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1508
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Canine
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
8-Hydroxy-2'-deoxyguanosine and 8-hydroxyguanosine.
Reactivity Notes
Due to the conserved nature of the target cross reactivity would be expected in wide range of species. Mouse reactivity reported in scientific literature (PMID: 29559852).
Localization
Nuclear
Specificity
NB600-1508 cross reacts completely with 8-oxo-dG, and does not cross react with other naturally occurring nucleotides.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Novus Biologicals Goat 8-oxo-dG Antibody (NB600-1508) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-8-oxo-dG Antibody: Cited in 18 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for 8-oxo-dG Antibody
ELISA: 8-OHdG Antibody [NB600-1508] - Competitive ELISA using the 8-OHG antibody demonstrates a high specificity towards 8-hydroxydeoxyguanosine (8-OHdG) and 8-hydroxyguanosine nucleotides. Partial cross-reactivity is observed with 8-mercaptoguanosine while 8-bromoguanosine shows some cross-reactivity. 2-Deoxyadenosine, 7-methylguanosine, guanosine monophosphate and guanosine show no reactivity.
Applications for 8-oxo-dG Antibody
Application
Recommended Usage
ELISA
1:100000-1:250000
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Application Notes
For IHC-Paraffin: It is suggested that the tissues are pretreated with 10 ug/mL of proteinase K for 40 minutes at 37 degrees C prior to staining. Dewaxed sections treated with 3%H2O2 in methanol for 30 minutes and blocking was with 10% serum in TBS. Sections were treated with proteinase K 10ug/ml in PBS pH 7.4, 40 minutes 37 degrees C prior to blocking. Detection is via DAB. Since the antibody is staining a nucleic acid it is important to use enough proteinase to penetrate the histone barrier.
Use in ICC/IF reported in scientific literature (PMID: 28269997).
Use in Western Blot reported in scientific literature (PMID:32269708).
Use in ICC/IF reported in scientific literature (PMID: 28269997).
Use in Western Blot reported in scientific literature (PMID:32269708).
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Preservative
0.01% Thimerosal
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 8-oxo-dG
The production of 8-Hydroxyguanine (8-oxo-dG) is almost exclusively elicited by oxidative stress. Polymerases preferentially insert adenine opposite 8-oxo-dG. Therefore, oxidatively damaged adducts, without repair, are susceptible to G to T transitions.
Long Name
8-Hydroxyguanine
Alternate Names
8-OHG, 8oxodG, 8-Hydroxyguanine
Additional 8-oxo-dG Products
Product Documents for 8-oxo-dG Antibody
Product Specific Notices for 8-oxo-dG Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.