Immunohistochemistry (IHC) Handbook

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This immunohistochemistry (IHC) handbook provides a detailed overview of the IHC procedure and its essential components, including step-by-step staining protocols and troubleshooting tips.

Immunohistochemistry (IHC) is a widely used immunostaining technique that detects an antigen of interest in the cells of a fixed tissue section, often analyzed with a light microscope.

This handbook provides a comprehensive introduction to IHC that is well-suited for researchers new to the technique or experienced scientists looking for a quick refresher.

Download the handbook and learn about:

  • Different variables to consider when performing an IHC experiment
  • Basic overview of an IHC experiment
  • Tissue sample preparation and fixation
  • Controls for IHC
  • Complementary techniques including RNA in situ hybridization (ISH)
  • How to optimize IHC workflow
  • Step-by-step IHC staining protocols and troubleshooting tips