Immunohistochemistry (IHC) is a widely used immunostaining technique that detects an antigen of interest in the cells of a fixed tissue section, often analyzed with a light microscope.
This handbook provides a comprehensive introduction to IHC that is well-suited for researchers new to the technique or experienced scientists looking for a quick refresher.
Download the handbook and learn about:
- Different variables to consider when performing an IHC experiment
- Basic overview of an IHC experiment
- Tissue sample preparation and fixation
- Controls for IHC
- Complementary techniques including RNA in situ hybridization (ISH)
- How to optimize IHC workflow
- Step-by-step IHC staining protocols and troubleshooting tips