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Immunohistochemistry (IHC) Handbook


E-books Summary

Immunohistochemistry (IHC) Handbook

Immunohistochemistry (IHC) is a widely used immunostaining technique using antibodies to detect an antigen of interest in the cells of a fixed tissue section and provides semi-quantitative data about target protein expression, distribution, and localization.

Our recently updated handbook offers a comprehensive introduction to IHC that is well-suited for researchers new to the technique and experienced scientists looking for a quick refresher.

Download our NEW handbook and learn about:

  • Different variables to consider when performing an IHC experiment
  • Multiplexing IHC
  • RNA-Protein co-detection and the benefits of in situ hybridization (ISH)-IHC
  • Standard IHC workflow including sample preparation and fixation, antigen retrieval, blocking non-specific binding, antibody selection, and detection methods
  • Controls for IHC
  • Easy-to-follow IHC staining protocols (chromogenic and fluorescent)
  • Troubleshooting tips
  • Buffer and reagent recipes

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