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Wnt Family

R&D Systems Recombinant Mouse Wnt-1/sFRP-1 Complex Activates Wnt Signaling

Assessment of the ability of R&D Systems Wnt/sFRP-1 complex to induce TCF reporter activity in HEK293 cells

R&D Systems Recombinant Mouse Wnt-1/sFRP-1 Complex Activates Wnt Signaling. The ability of Recombinant Mouse Wnt-1/sFRP-1 Complex (R&D Systems, Catalog # 9765-WN) to stimulate beta-Catenin activation was tested in the HEK293 human embryonic kidney cell line using a TOPflash beta-Catenin/TCF reporter assay. The ED50 for this effect is 0.03-0.18 ug/mL.  

Immunostaining of Wnt-2 in Human Stomach Cancer Tissue Using R&D Systems Goat Anti-Human Wnt-2 Antigen Affinity-Purified Polyclonal Antibody

Detection of Wnt-2 in human stomach cancer tissue using an anti-human Wnt-2 antigen affinity-purified polyclonal antibody

Detection of Wnt-2 in Human Stomach Cancer Tissue. Wnt-2 was detected in immersion-fixed, paraffin-embedded sections of human stomach cancer tissue using a Goat Anti-Human Wnt-2 Antigen Affinity-purified Polyclonal Antibody (R&D Systems, Catalog # AF3464) overnight at 4 C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (R&D Systems, Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R&D Systems, Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

R&D Systems Recombinant Human Wnt-3a Displays Higher Activity than a Leading Competitor’s Recombinant Human Wnt-3a and Shows Minimal Lot-to-Lot Variability

Assessment of the bioactivity and lot-to-lot consistency of R&D Systems recombinant human Wnt-3a protein

R&D Systems Recombinant Human Wnt-3a Displays Higher Activity than a Leading Competitor’s Recombinant Human Wnt-3a Protein and Shows Minimal Lot-to-Lot Variability (A) The bioactivity of R&D Systems Recombinant Human Wnt-3a (Catalog # 5036-WN; orange line) or recombinant human Wnt-3a from another company (green line) was assessed by measuring the ability of the proteins to induce alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. The ED50 of R&D Systems Recombinant Human Wnt-3a was 1.7-fold better than the leading competitor’s Wnt-3a protein, with more than twice the maximum response. (B) Three independent lots of R&D Systems Recombinant Human Wnt-3a (Catalog # 5036-WN) were tested for their ability to induce alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. Each trace shown on the graph represents data obtained from Recombinant Human Wnt-3a from a different manufacturing run.

Immunostaining of Frizzled-7 in the HEK293 Human Embryonic Kidney Cell Line Using R&D Systems Rat Anti-Human/Mouse Frizzled-7 Monoclonal Antibody

Detection of Frizzled-7 in HEK293 cells using R&D Systems rat anti-human/mouse Frizzled-7 monoclonal antibody

Detection of Frizzled-7 in the HEK293 Human Embryonic Kidney Cell Line. Frizzled-7 was detected in immersion-fixed HEK293 human embryonic kidney cells using a Rat Anti-Human/Mouse Frizzled-7 Monoclonal Antibody (R&D Systems, Catalog # MAB1981) for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (R&D Systems, Catalog # NL013; red) and counterstained with DAPI (Tocris, Catalog # 5748; blue). Specific staining was localized to the cell surface.

Wnt Reporter Activity Induced by R&D Systems Recombinant Human Wnt-3a and Inhibition by Recombinant Human Dkk-1

Wnt-3a-induced TCF reporter activity and neutralization by R&D Systems recombinant human Dkk-1

R&D Systems Recombinant Human Dkk-1 Inhibits Wnt-3a-Induced beta-Catenin/TCF Reporter Activity. The ability of Recombinant Human Wnt-3a (R&D Systems, Catalog # 5036-WN) to stimulate beta-Catenin activation was tested in the HEK293 human embryonic kidney cell line using a TOPflash beta-Catenin/TCF reporter assay (green line). The ED50 for this effect is <500 ng/mL. The effect induced by 500 ng/mL Recombinant Human Wnt-3a was inhibited by treating the cells with increasing concentrations of Recombinant Human Dkk-1 (R&D Systems, Catalog # 5439-DK; orange line). The ED50 for this effect is 10-60 ng/mL.

R&D Systems Recombinant Human R-Spondin 1 Displays Higher Activity than a Leading Competitor’s Recombinant Human R-Spondin 1 and Shows Minimal Lot-to-Lot Variability

Assessment of the bioactivity and lot-to-lot consistency of R&D Systems recombinant human R-Spondin 1 protein

R&D Systems Recombinant Human R-Spondin 1 Displays Higher Activity than a Leading Competitor’s Recombinant Human R-Spondin 1 Protein and Shows Minimal Lot-to-Lot Variability. (A) The bioactivity of R&D Systems Recombinant Human R-Spondin 1 (Catalog # 4645-RS; blue line) or recombinant human R-Spondin 1 from another company (purple line) was assessed by measuring the ability of the proteins to stimulate beta-Catenin activation in the HEK293T human kidney cell line using a TOPflash beta-Catenin/TCF reporter assay, in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (R&D Systems, Catalog # 1324-WN). The ED50 for this effect for R&D Systems Recombinant Human R-Spondin 1 was approximately 7-fold greater than the leading competitor’s R-Spondin 1 protein. (B) Three independent lots of R&D Systems Recombinant Human R-Spondin 1 (Catalog # 4645-RS) were tested for their ability to stimulate beta-Catenin activation in the HEK293T human kidney cell line using a TOPflash beta-Catenin/TCF reporter assay, in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (R&D Systems; Catalog # 1324-WN). Each trace shown on the graph represents data obtained from Recombinant Human R-Spondin 1 from a different manufacturing run.

Featured Wnt Family-Related Products

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Featured Resources for Wnt Family Research

Thumbnail image of the cover page from the white paper, The First Bioactive Wnt1 Protein

White Paper: The First Bioactive Wnt1 Protein

Plasma membrane tethering has made the purification of bioactive Wnt-1 protein technically challenging. Read this white paper to find out how scientists at R&D Systems purified Wnt1/sFRP complexes to bring to market the first commercially available bioactive Wnt1 protein.

 The beta-Catenin-dependent Wnt signaling pathway is initiated by association of Wnt with the Frizzled and LRP-5/6 receptors

Growth Factor Signaling Pathways

Growth factors activate intracellular signaling pathways that regulate a diverse range of biological processes, including development, cell proliferation, differentiation, motility, angiogenesis, and adult tissue homeostasis. Explore the signaling pathways that are activated by different growth factor families and the biological effects that they mediate using our interactive signaling pathways.

Thumbnail image of the application note, Optimizing Organoid Culture Conditions

Application Note: Optimizing Organoid Culture Conditions

Establishing and optimizing the appropriate conditions for culturing different types of organoids can be technically challenging. Read this application note to learn about the importance of growth factor bioactivity and reagent consistency for achieving robust, reproducible organoid growth.