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Cultrex™ UltiMatrix RGF Basement Membrane Extract Sample Request

What is Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract?

Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract is a new, specially formulated, soluble extracellular matrix hydrogel that is designed to improve the performance and consistency of 2-D and 3-D cell cultures. Features of this matrix include an elevated protein concentration, enhanced levels of entactin, high tensile strength, and robust clarity and purity. Cultrex UltiMatrix RGF Basement Membrane Extract is qualified for organoid cell culture, spheroid formation, and embryonic or induced pluripotent stem cell expansion and maintenance, along with other 2-D and 3-D cell culture applications.

Our development scientists have rigorously tested Cultrex UltiMatrix RGF Basement Membrane Extract so that researchers can be confident that their culture conditions will be reproducible from one experiment to the next. Cultrex UltiMatrix RGF Basement Membrane Extract supports the growth of both robust and difficult to culture organoids, ultra-low adhesion embedding for spheroids, and thin-layer coating for the expansion and maintenance of embryonic stem cells and iPSCs. Side-by-side comparison testing was also performed with the leading commercial basement membrane extract and demonstrated that Cultrex UltiMatrix RGF Basement Membrane Extract has more consistent protein concentrations and gelling rates across lots.

Request a free sample of this new optimized matrix and see for yourself how Cultrex UltiMatrix RGF Basement Membrane Extract can improve the performance and consistency of your cultures.

Images of Organoids Cultured Using Cultrex UltiMatrix RGF Basement Membrane Extract

Human lung organoids grown in Cultrex UltiMatrix RGF BME and stained for Cytokeratin 5, p63, Podoplanin, Muc5a, and SOX2.

Human Lung Organoids Grown in Cultrex UltiMatrix RGF Basement Membrane Extract. Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (R&D SystemsTM, Catalog # BME001-05) and cultured in media for 20-60 days. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. A) Lung organoids were stained with anti-Cytokeratin 5 (KRT5) (green; Novus BiologicalsTM, Catalog # NB110-56916) and with anti-p63/TP73L (red; R&D Systems, Catalog # AF1916) to visualize basal cells. B) Lung organoids were stained with anti-Podoplanin (PDPN) (green; Novus Biologicals, Catalog # NB600-1015) to visualize alveolar type I cells. C, D) Lung organoids were stained with anti-Muc5ac (green; Novus Biologicals, Catalog # NBP2-15196) to visualize Goblet cells and with anti-Sox2 (red; R&D Systems, Catalog # MAB2018). All samples were counterstained with the nuclear stain DAPI (blue; Tocris, catalog # 5748).

Cerebral Organoids Grown in Cultrex UltiMatrix RGF Basement Membrane Extract. iPSC-derived cerebral organoids (day 45) were cultured using Cultrex UltiMatrix RGF Basement Membrane Extract (R&D Systems, Catalog # BME001-05) and stained for Syto16 (blue), Pax6 (red), and Vimentin (green). A) Images taken at 4x magnification. B) An enlarged view of the area shown within the white box in part A of the figure. C) Images taken at 15x magnification. Images courtesy of LifeCanvas Technologies.

Background Information

Cultrex UltiMatrix Sample Request

Terms & Conditions: No purchase necessary. This promotion is available in the US, Canada, and Europe. Sample requests will be honored while supplies last. The sample is the Cultrex UltiMatrix RGF BME (Catalog # BME001-01). Limit one free sample per customer. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.