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PA Janelia Fluor® 646, SE

Photoactivatable Dyes Small Molecule
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Description: Red photoactivatable dye (Reactive group: NHS ester). Applications: super resolution microscopy (PALM), single molecule tracking and live cell imaging
Chemical Name: 2,5-Dioxo-1-pyrrolidinyl 3,7-di-1-azetidinyl-2'-diazo-5,5-dimethyl-2',3'-dihydro-5H-3'-oxospiro[dibenzo[b,e]siline-10,1'-indene]-6'-carboxylate
Purity: ≥90% (HPLC)
Molecular Weight: 617.74

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  • PA-JF646 for tracking single recombinant proteins
    Name: Anonymous
    Species: Other
    Assay Type: In Vitro
    This product was used to label recombinant proteins and image them with single molecule sensitivity. The degree of labeling achieved was 1.4 (average). The photoactivation efficiency of the dye is excellent and can be used to track a few molecules at a time, for long periods. The dye is highly photostable and can be used to track proteins for ~10-15 seconds under ideal tracking conditions. I attach here an image before and after photoactivation of the dye. Upon photoactivation, single dye molecules conjugated to the protein are visible.
    PA Janelia Fluor® 646, SE Serum 6150

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