Calpeptin

Name: Calpeptin
Brand: Tocris Bioscience
SKU: 0448
Price: 197 USD

Tocris Bioscience a Bio-Techne Brand

Catalog # 0448

Citations (10)

COA / SDS

Catalog #

Availability

Size / Price

Qty

Key Product Details

Description:	Calpain and cathepsin L inhibitor
Chemical Name:	N-Benzyloxycarbonyl-L-leucylnorleucinal
Molecular Weight:	362.47

View Full Technical Details for Calpeptin

Biological Activity for Calpeptin

Calpeptin is a potent, cell-permeable inhibitor of the Ca²⁺-dependent protease, calpain. Prevents collagen- and thrombin-induced platelet aggregation, probably by blocking calpain induced phospholipase C and thromboxane synthase activation. Potent cathepsin L inhibitor. Recently shown to preferentially inhibit a subset of protein-tyrosine phosphatases. Also inhibits SARS-CoV-2 M^pro in vitro (IC₅₀ = 10.7 μM) and inhibits viral replication.

Technical Data for Calpeptin

M. Wt	362.47
Formula	C₂₀H₃₀N₂O₄
Storage	Desiccate at +4°C
CAS Number	117591-20-5
PubChem ID	73364
InChi Key	PGGUOGKHUUUWAF-ROUUACIJSA-N
Smiles	O=C(OCC1=CC=CC=C1)N[C@H](C(N[C@H](C([H])=O)CCCC)=O)CC(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Calpeptin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO		100

Preparing Stock Solutions for Calpeptin

The following data is based on the product molecular weight 362.47.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.76 mL	13.79 mL	27.59 mL
5 mM	0.55 mL	2.76 mL	5.52 mL
10 mM	0.28 mL	1.38 mL	2.76 mL
50 mM	0.06 mL	0.28 mL	0.55 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Calpeptin

References are publications that support the biological activity of the product.

Ma Boceprevir, GC-376, and calpain inhibitors II, XII inhibit SARS-CoV-2 viral replication by targeting the viral main protease. Cell Res. 2020 PMID: 32541865
Schoenwaelder and Burridge Evidence for a calpeptin-sensitive protein-tyrosine phosphatase upstream of the small GTPase Rho. J.Biol.Chem. 1999 PMID: 10318859
Mehdi Cell-penetrating inhibitors of calpain. TiBS 1991 PMID: 1877091
Sasaki Inhibitory effect of di-and tripeptidyl aldehydes on calpains and cathepsins. J.Enzyme Inhib. 1990 PMID: 2079636
Tsujinaka Synthesis of a new cell penetrating calpain inhibitor (calpeptin). Biochem.Biophys.Res.Commun. 1988 PMID: 2839170

Citations for Calpeptin (10)

Showing 1 - 10 of 10 citations Showing All

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Calpeptin include:

Zhou and Cai (2019) Calpeptin Reduces Neurobehavioral Deficits and Neuronal Apoptosis Following Subarachnoid Hemorrhage in Rats. J Stroke Cerebrovasc Dis. 28:125
PMID: 30337208
Rivera-Pagán et al. (2015) Up-regulation of TREK-2 potassium channels in cultured astrocytes requires de novo protein synthesis: relevance to localization of TREK-2 channels in astrocytes after transient cerebral ischemia. PLoS One. 10:e0125195
PMID: 25886567
Machado et al. (2015) Involvement of calpains in adult neurogenesis: implications for stroke. J Biol Chem. 9:22
PMID: 25698931
Wang et al. (2015) MFN2 couples glutamate excitotoxicity and mitochondrial dysfunction in motor neurons. J Pharmacol Exp Ther. 290:168
PMID: 25416777
Zakharova et al. (2015) Platelet surface-associated activation and secretion-mediated inhibition of coagulation factor XII. PLoS One. 10:e0116665
PMID: 25688860
Redpath et al. (2014) Calpain cleavage within dysferlin exon 40a releases a synaptotagmin-like module for membrane repair. Mol Biol Cell. 25:3037
PMID: 25143396
Chen et al. (2013) Nerve injury increases GluA2-lacking AMPA receptor prevalence in spinal cords: functional significance and signaling mechanisms. Pflugers Arch. 347:765
PMID: 24030012
Santos et al. (2012) Distinct regulatory functions of calpain 1 and 2 during neural stem cell self-renewal and differentiation. PLoS One. 7:e33468
PMID: 22432027
Leytin et al. (2009) Mitochondrial control of platelet apoptosis: effect of cyclosporin A, an inhibitor of the mitochondrial permeability transition pore. Lab Invest. 89:374
PMID: 19238135
Amadoro et al. (2006) NMDA receptor mediates tau-induced neurotoxicity by calpain and ERK/MAPK activation. Proc Natl Acad Sci U S A. 103:2892
PMID: 16477009

There are no citations that match your criteria.